Tanaka H, Nishida T
J Cell Physiol. 1985 May;123(2):191-6. doi: 10.1002/jcp.1041230206.
To understand corneal wound healing, in which fibronectin plays an important role, we investigated the regulatory mechanism of fibronectin synthesis in cultured rabbit corneal blocks in situ. The amount of fibronectin was determined by enzyme-linked immunosorbent assay. We found that butyrate stimulated fibronectin synthesis in a dose-response fashion, and that butyrate had a greater stimulatory effect than did any of its derivatives examined. This suggests that butyrate stimulates fibronectin synthesis specifically; 8Br-cAMP also stimulated fibronectin synthesis. Additivity of stimulation by butyrate and by 8Br-cAMP was observed at the saturated concentration of each; the present result indicated an independent mechanism of action for these two compounds.
为了解纤连蛋白在其中发挥重要作用的角膜伤口愈合过程，我们研究了培养的兔角膜块原位纤连蛋白合成的调控机制。通过酶联免疫吸附测定法测定纤连蛋白的含量。我们发现丁酸盐以剂量反应方式刺激纤连蛋白合成，并且丁酸盐比所检测的任何一种其衍生物具有更大的刺激作用。这表明丁酸盐特异性地刺激纤连蛋白合成；8-溴环磷酸腺苷（8Br-cAMP）也刺激纤连蛋白合成。在每种化合物的饱和浓度下观察到丁酸盐和8Br-cAMP刺激的相加作用；目前的结果表明这两种化合物具有独立的作用机制。
