Nishida T, Tanaka H, Nakagawa S, Sasabe T, Awata T, Manabe R
Jpn J Ophthalmol. 1984;28(2):196-202.
We investigated whether the cornea can synthesize fibronectin which participates in corneal wound-healing. We also examined the effects of epidermal growth factor (EGF) and cyclic AMP analogs on fibronectin synthesis by the cornea. Rabbit corneal blocks were cultured in medium without serum, and the amount of fibronectin in culture medium was determined by ELISA assay. When rabbit corneal blocks were cultured in the medium alone, fibronectin contents in the medium increased with increase in the incubation period. This increase was enhanced by the addition of EGF to the culture medium, and was inhibited by addition of a protein synthesis inhibitor, cycloheximide. The addition of cAMP analogs, 8-bromo cyclic-AMP and dibutyryl cAMP, to the culture medium also increased the rate of fibronectin production by the cornea. These results show that the cornea can synthesize fibronectin and its synthesis is stimulated by EGF and cAMP analogs.
我们研究了角膜是否能够合成参与角膜伤口愈合的纤连蛋白。我们还检测了表皮生长因子（EGF）和环磷酸腺苷类似物对角膜纤连蛋白合成的影响。将兔角膜块在无血清培养基中培养，通过酶联免疫吸附测定法（ELISA）测定培养基中纤连蛋白的含量。当兔角膜块单独在培养基中培养时，培养基中纤连蛋白的含量随培养时间的增加而增加。向培养基中添加EGF可增强这种增加，而添加蛋白质合成抑制剂环己酰亚胺则可抑制这种增加。向培养基中添加环磷酸腺苷类似物8-溴环磷酸腺苷和二丁酰环磷酸腺苷也可提高角膜纤连蛋白的产生率。这些结果表明，角膜能够合成纤连蛋白，并且其合成受到EGF和环磷酸腺苷类似物的刺激。
