Bege Jonathan, Pang Wei Quan, Chew Bee Lynn, Sivalingam Elayabalan, Manickam Sankar, Mad' Atari Mohamad Fadhli, Subramaniam Sreeramanan
School of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Georgetown, Penang, Malaysia.
Department of Biochemistry, Faculty of Natural and Applied Sciences, Plateau State University, Bokkos, Nigeria.
Photosynth Res. 2025 Jan 13;163(1):4. doi: 10.1007/s11120-024-01130-5.
This study examined the impacts of different LED spectra on the growth of in vitro cultures of Musa acuminata cv. red banana and their biochemical profile, including the antioxidant enzymes catalase and ascorbate peroxidase, photosynthetic pigment and accumulation of total carbohydrate content. The far-red LEDs significantly increase shoot elongation (10.04 cm). The greatest number of shoots (2.97) and the greatest multiplication rate (80%) were obtained under the treatment with blue + red LEDs. The formation of microshoots were also enhanced by blue and white LED exposure in a range of 2-2.57 shoots per explant. Root formation was also stimulated by dichromatic blue + red (6.00) LED using MS medium with 2 µM indole-3-butyric acid (IBA). The enzymes catalase and ascorbate peroxidase were significantly up-regulated by irradiation with far-red (0.11 ± 0.02 CAT, 0.18 ± 0.04 APX U/mg) and blue (0.08 ± 0.01CAT, 0.10 ± 0.01APX U/mg) LED light. Total chlorophyll (0.45 to 0.80 mg/g) was elevated significantly by blue, blue + red and mint-white LED. On the other hand, carotenoids (12.08-14.61 mg/g) were significantly boosted by blue + red, red and mint-white LED light. Meanwhile, porphyrin (294.10-350.57 mg/g) was highly synthesised after irradiation with mint-white light. Irradiation with LED light significantly increased the accumulation of carbohydrates with the highest carbohydrate content under blue + red LED light (102.22 ± 2.46 mg/g) and blue light (91.69 ± 2.10 mg/g). In conclusion, these results confirm that the vegetative properties and biochemical profile of red banana in vitro are eustress response to LED spectra.
本研究考察了不同LED光谱对尖叶蕉品种红香蕉离体培养物生长及其生化特征的影响，包括抗氧化酶过氧化氢酶和抗坏血酸过氧化物酶、光合色素以及总碳水化合物含量的积累。远红光LED显著促进了茎尖伸长（10.04厘米）。在蓝光+红光LED处理下，获得了最多的茎芽数量（2.97个）和最高的增殖率（80%）。蓝光和白光处理也增强了微芽的形成，每个外植体形成2 - 2.57个芽。使用添加2 μM吲哚-3-丁酸（IBA）的MS培养基，双色蓝光+红光（6.00个）LED也刺激了根系形成。过氧化氢酶和抗坏血酸过氧化物酶在远红光（0.11±0.02 CAT，0.18±0.04 APX U/mg）和蓝光（0.08±0.01 CAT，0.10±0.01 APX U/mg）照射下显著上调。蓝光、蓝光+红光和薄荷白光LED显著提高了总叶绿素含量（0.45至0.80毫克/克）。另一方面，蓝光+红光、红光和薄荷白光LED显著提高了类胡萝卜素含量（12.08 - 14.61毫克/克）。同时，薄荷白光照射后卟啉高度合成（294.10 - 350.57毫克/克）。LED光照显著增加了碳水化合物的积累，蓝光+红光LED光照下碳水化合物含量最高（102.22±2.46毫克/克），蓝光下为（91.69±2.10毫克/克）。总之，这些结果证实了红香蕉离体培养物的营养特性和生化特征是对LED光谱的良性应激反应。
