Costa Érica Letícia Gomes, Farnese Fernanda Dos Santos, de Oliveira Thales Caetano, Rosa Márcio, Rodrigues Arthur Almeida, Resende Erika Crispim, Januario Ana Helena, Silva Fabiano Guimarães
Departamento de Biotecnologia, Instituto Federal de Educação, Ciência e Tecnologia Goiano, Rio Verde, Brazil.
Laboratório de Fisiologia Vegetal, Instituto Federal de Educação, Ciência e Tecnologia Goiano, Rio Verde, Brazil.
Front Plant Sci. 2021 Jul 21;12:680545. doi: 10.3389/fpls.2021.680545. eCollection 2021.
is a plant species with medicinal relevance due to its furanocoumarin accumulation. The accumulation of these compounds in the root promotes predatory extractivism, which threatens the conservation of the species. In addition, little is known about the conditions for culturing of this species . The present study aimed to investigate how the application of different spectra of LEDs (white, blue, red, and combinations of blue and red at 1:1 and 3:1 ratios) can impact the morphophysiological and biochemical characteristics of under different conditions. To evaluate the production of furanocoumarins in its leaves, which are easy-to-collect perennial organs, we cultured nodal segments in 50-mL tubes with MS medium under 100 μmol m s light and a photoperiod of 16 h for 50 days. We then submitted the seedlings biometric, anatomical, biochemical, and physiological evaluations. The different spectral qualities influenced several characteristics of the seedlings. Plants grown under red light showed greater stem elongation and larger and thinner leaves, strategies aimed at capturing a higher ratio of radiant energy. Exposure to the blue/red ratio of 1:1 induced increases in the concentration of the furanocoumarin psoralen, probably due to the diversion of carbon from primary metabolism, which resulted in lower growth. Cultivation under blue light or blue:red light at 3:1 triggered anatomical and physiological changes that led to higher production of secondary metabolites in the leaves, and at the 3:1 ratio, the seedlings also had a high growth rate. These results highlight the fundamental role of light in stimulating the production of secondary metabolites, which has important implications for the production of compounds of interest and indirect consequences for the conservation of .
由于其呋喃香豆素的积累，是一种具有药用价值的植物物种。这些化合物在根部的积累促进了掠夺性采集，这对该物种的保护构成了威胁。此外，关于该物种的培养条件知之甚少。本研究旨在探讨不同光谱的发光二极管（白色、蓝色、红色以及蓝色和红色以1:1和3:1比例组合）的应用如何在不同条件下影响该植物的形态生理和生化特性。为了评估其叶片（易于采集的多年生器官）中呋喃香豆素的产生，我们将节段在装有MS培养基的50毫升试管中培养，光照强度为100 μmol m² s⁻²，光周期为16小时，持续50天。然后我们对幼苗进行了生物统计学、解剖学、生化和生理学评估。不同的光谱质量影响了幼苗的几个特征。在红光下生长的植物表现出更大的茎伸长以及更大更薄的叶子，这是旨在捕获更高比例辐射能的策略。暴露于1:1的蓝/红比例会导致呋喃香豆素补骨脂素浓度增加，这可能是由于碳从初级代谢中转移，从而导致生长减缓。在蓝光或3:1的蓝/红光下培养会引发解剖学和生理学变化，导致叶片中次生代谢产物的产量更高，并且在3:1的比例下，幼苗也具有较高的生长速率。这些结果突出了光在刺激次生代谢产物产生中的基本作用，这对感兴趣化合物的生产具有重要意义，并对该植物的保护产生间接影响。
