ADAMTS-2在系统性红斑狼疮中的表达及其临床意义分析
Analysis of expression and its clinical significance of the ADAMTS-2 in systemic lupus erythematosus.
作者信息
Long Dan, Zhan Jiahuan, Tu Chaofei, Yu Shujiao, Rao Jiayue, Luo Qing, Huang Zikun, Li Junming
机构信息
Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, No. 17 Yongwaizheng Street, Donghu District, Nanchang City, 330000, Jiangxi, China.
出版信息
Clin Rheumatol. 2025 Feb;44(2):681-691. doi: 10.1007/s10067-025-07303-4. Epub 2025 Jan 14.
OBJECTIVES
ADAMTS-2 is a procollagen N-proteinase that plays an important role in inflammation regulation. The objective of our research is to explore the expression of ADAMTS-2 in Systemic Lupus Erythematosus (SLE), and analyze its relationship with clinical features of SLE, and evaluate the potential value of ADAMTS-2 as a diagnostic biomarker in SLE.
METHODS
ADAMTS-2 expression in PBMCs was detected by RT-qPCR in SLE patients, RA patients, and healthy controls (HC). The diagnostic value of ADAMTS-2 for SLE was evaluated by ROC curve, and the correlation between ADAMTS-2 and the clinical characteristics of SLE was analyzed by Spearman's rank correlation coefficient. The expression profiles of GSE8650 and GSE82221 were downloaded from the GEO database. We performed GSEA to further understand the functions of ADAMTS-2 in SLE. CIBERSORT was utilized for immune cell infiltration analysis.
RESULTS
RT-qPCR results validated that the expression of ADAMTS-2 in PBMCs was significantly increased in SLE patients than RA patients and HC. ROC anaylsis suggested that ADAMTS-2 has significant value in distinguishing new-onset SLE patients from RA patients and HC (AUC = 0.805, p < 0.0001). The expression of ADAMTS-2 was negatively correlated with C3, WBC, PLT, neutrophil, and monocyte level. PBMCs samples with high ADAMTS-2 expression were enriched in TNFA_SIGNALING_VIA_NFKB pathway. We found that ADAMTS-2 was positively correlated with neutrophils, M0 macrophages and M2 macrophages.
CONCLUSION
ADAMTS-2 may be a potential biomarker of SLE patients and closely related to the occurrence and development of SLE. ADAMTS-2 is expected to be a new target for SLE treatment. Key Points • ADAMTS-2 is a potential biomarker of disease activity in SLE patients that develop a flare. • Samples with high ADAMTS-2 expression are enriched in TNFA_SIGNALING_VIA_NFKB pathway in SLE. • ADAMTS-2 expression is positively correlated with neutrophils, M0 macrophages and M2 macrophages.
目的
ADAMTS - 2是一种原胶原蛋白N蛋白酶，在炎症调节中起重要作用。本研究的目的是探讨ADAMTS - 2在系统性红斑狼疮（SLE）中的表达，分析其与SLE临床特征的关系，并评估ADAMTS - 2作为SLE诊断生物标志物的潜在价值。
方法
采用RT - qPCR检测SLE患者、类风湿关节炎（RA）患者和健康对照（HC）外周血单个核细胞（PBMCs）中ADAMTS - 2的表达。通过ROC曲线评估ADAMTS - 2对SLE的诊断价值，采用Spearman等级相关系数分析ADAMTS - 2与SLE临床特征的相关性。从GEO数据库下载GSE8650和GSE82221的表达谱。我们进行基因集富集分析（GSEA）以进一步了解ADAMTS - 2在SLE中的功能。利用CIBERSORT进行免疫细胞浸润分析。
结果
RT - qPCR结果证实，SLE患者PBMCs中ADAMTS - 2的表达显著高于RA患者和HC。ROC分析表明，ADAMTS - 2在区分新发SLE患者与RA患者和HC方面具有显著价值（AUC = 0.805，p < 0.0001）。ADAMTS - 2的表达与C3、白细胞、血小板、中性粒细胞和单核细胞水平呈负相关。ADAMTS - 2表达高的PBMCs样本在TNFA_SIGNALING_VIA_NFKB通路中富集。我们发现ADAMTS - 2与中性粒细胞、M0巨噬细胞和M2巨噬细胞呈正相关。
结论
ADAMTS - 2可能是SLE患者的潜在生物标志物，与SLE的发生发展密切相关。ADAMTS - 2有望成为SLE治疗的新靶点。要点：•ADAMTS - 2是发生病情 flare 的SLE患者疾病活动的潜在生物标志物。•SLE中ADAMTS - 2表达高的样本在TNFA_SIGNALING_VIA_NFKB通路中富集。•ADAMTS - 2表达与中性粒细胞、M0巨噬细胞和M2巨噬细胞呈正相关。
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