Adhikari Pratikshya, Xu Hao
Gene Therapy Center, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Center for Molecular and Cellular Biosciences, School of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, MS, USA.
Methods Mol Biol. 2025;2887:149-166. doi: 10.1007/978-1-0716-4314-3_10.
SNARE-dependent mast cell (MC) exocytosis causes the release of a wide variety of mediators with important physiological/pathological consequences. Unlike synaptic transmission in the brain, which relies primarily on one set of exocytic SNAREs (i.e., Syntaxin1, SNAP-25, and VAMP2), MCs produce a multitude of exocytic SNAREs that can form a minimum of 8 distinct sets of fusogenic trans-SNARE complexes. Here we describe the genetic approaches we have developed to dissect the specific roles of these SNAREs in RBL-2H3 cells, a widely utilized model for studying MC signaling and exocytosis.
依赖SNARE的肥大细胞（MC）胞吐作用会导致多种介质的释放，产生重要的生理/病理后果。与主要依赖一组胞吐SNARE（即Syntaxin1、SNAP - 25和VAMP2）的大脑突触传递不同，肥大细胞产生多种胞吐SNARE，它们可以形成至少8种不同的融合性反式SNARE复合物。在这里，我们描述了我们开发的遗传方法，以剖析这些SNARE在RBL - 2H3细胞中的特定作用，RBL - 2H3细胞是研究肥大细胞信号传导和胞吐作用广泛使用的模型。
