Moo-Penn W F, Jue D L
J Chromatogr. 1985 Jan 18;318(2):325-32. doi: 10.1016/s0021-9673(01)90693-4.
Our studies on the separation of hemoglobin variants using Monobead ion exchangers show that these ion exchangers will resolve variants that heretofore could not be resolved. Procedures with these ion exchangers, therefore, extend our ability to detect hemoglobinopathies. Since it is also important to study the physicochemical properties of variants, their isolation is of critical importance to the researcher. The methods described in this report enable variants to be isolated in quantitative amounts so that such studies can be performed. Most isolations can be accomplished within 1 h, under mild conditions with aqueous buffers. Elution gradients can be changed conveniently to optimize resolution of most variants. These methods should serve as a valuable adjunct in studying the structure-function relationship of hemoglobin and other proteins.
我们使用单珠离子交换剂分离血红蛋白变体的研究表明，这些离子交换剂能够分离出此前无法分离的变体。因此，使用这些离子交换剂的方法扩展了我们检测血红蛋白病的能力。由于研究变体的物理化学性质也很重要，其分离对研究人员至关重要。本报告中描述的方法能够定量分离变体，从而可以进行此类研究。大多数分离可以在1小时内完成，在温和条件下使用水性缓冲液。洗脱梯度可以方便地改变，以优化大多数变体的分离效果。这些方法应成为研究血红蛋白和其他蛋白质结构-功能关系的有价值辅助手段。
