Van den Eeckhout E, De Moerloose P, Sinsheimer J E
J Chromatogr. 1985 Jan 18;318(2):343-9. doi: 10.1016/s0021-9673(01)90695-8.
A simple, rapid and sensitive high-performance thin-layer chromatographic assay for the determination of epoxide hydrolase activity in rat liver homogenates is described. It is extended to the determination of a series of phenoxypropane-1,2-diols. The hydrolase assay has the advantages of using a readily available substrate, 2,3-epoxypropyl 4-methoxyphenyl ether, of multiple sample application, and of the simultaneous determination of reaction product (diol) as well as unchanged substrate (epoxide). The use of an internal standard, 4-nitroanisole, results in high sensitivity and good reproducibility of the proposed method. The limit of diol detection is 20 pmol.
本文描述了一种用于测定大鼠肝脏匀浆中环氧化物水解酶活性的简单、快速且灵敏的高效薄层色谱分析法。该方法可扩展用于一系列苯氧基丙烷 -1,2 -二醇的测定。水解酶分析方法具有以下优点：使用易于获得的底物2,3 -环氧丙基4 -甲氧基苯基醚、可多次进样，以及能同时测定反应产物（二醇）和未反应的底物（环氧化物）。使用内标物4 -硝基苯甲醚可使该方法具有高灵敏度和良好的重现性。二醇的检测限为20皮摩尔。
