Sotnichenko A I, Serdiuk O A, Starovoĭtov M L, Parfenov E A, Sukhanov V A
Antibiot Med Biotekhnol. 1985 Jul;30(7):535-8.
A new method for determination of the activity of epoxide hydrolase (EC 3.3.2.3), an enzyme of the 2nd phase of the system of drug metabolism, in rat liver microsomes was developed. 9,10-Epoxy-9,10-dihydrophenanthrene was used as a substrate. The quantitative determination of 9,10-dioxy-9,10-dihydrophenanthrene was performed according to the method of the internal standard by HPLC in aqueous a cetonitrile on octylsilica gel with fluorescent detection. 1-Naphthol was used as the internal standard. The method is simple and requires no preliminary extraction of the sample and preparation of the derivatives. It is highly sensitive and rapid: the time of the chromatographic analysis is not more than 3 minutes. The error does not exceed 5 per cent.
开发了一种测定大鼠肝微粒体中环氧水解酶（EC 3.3.2.3）活性的新方法，环氧水解酶是药物代谢系统第二阶段的一种酶。以9,10-环氧-9,10-二氢菲为底物。采用内标法，在乙腈水溶液中，于辛基硅胶上，通过高效液相色谱荧光检测法对9,10-二羟基-9,10-二氢菲进行定量测定。以1-萘酚作为内标。该方法简单，无需对样品进行预提取和衍生物制备。它具有高灵敏度和快速性：色谱分析时间不超过3分钟。误差不超过5%。
