Svendsen J S, Whist J E, Sydnes L K
J Chromatogr. 1985 Jan 11;337(1):9-19. doi: 10.1016/0378-4347(85)80002-5.
A method involving derivatization and combined gas chromatography--mass spectrometry has been developed to separate the enantiomers of 3-hydroxyadipic acid. By combining this method with asymmetric synthesis of the same acid, it has been shown that 3-hydroxyadipic acid excreted in urine consists of at least 95% of the L-enantiomer. This finding supports the hypothesis that dicarboxylic acids are degraded by ordinary beta-oxidation, and indicates that adipic acid may be converted into succinic acid.
已开发出一种涉及衍生化和气相色谱 - 质谱联用的方法来分离3 - 羟基己二酸的对映体。通过将该方法与相同酸的不对称合成相结合，已表明尿液中排泄的3 - 羟基己二酸至少由95%的L - 对映体组成。这一发现支持了二羧酸通过普通β - 氧化降解的假说，并表明己二酸可能转化为琥珀酸。
