Marcell P D, Stabler S P, Podell E R, Allen R H
Anal Biochem. 1985 Oct;150(1):58-66. doi: 10.1016/0003-2697(85)90440-3.
Methylmalonic acid, succinic acid, and other dicarboxylic acids have been extracted and partially purified from serum and urine using ether extraction and high-performance liquid chromatography. The t-butyldimethylsilyl derivatives were prepared and analyzed using capillary gas chromatography-mass spectrometry with selected ion monitoring. The addition of [methyl-2H3]methylmalonic acid and [1,4-13C2]succinic acid to the starting samples made it possible to quantitate these two dicarboxylic acids. Normal ranges for methylmalonic acid and succinic acid were determined in human and rat serum and in human urine. The utilization of other internal standards would make it possible to quantitate malonic, dimethylmalonic, ethylmalonic, methylsuccinic, glutaric, and other dicarboxylic acids.
已使用乙醚萃取和高效液相色谱法从血清和尿液中提取并部分纯化了甲基丙二酸、琥珀酸和其他二羧酸。制备了叔丁基二甲基甲硅烷基衍生物，并使用具有选择离子监测功能的毛细管气相色谱-质谱联用仪进行分析。向起始样品中添加[甲基-2H3]甲基丙二酸和[1,4-13C2]琥珀酸使得定量这两种二羧酸成为可能。测定了人和大鼠血清以及人尿液中甲基丙二酸和琥珀酸的正常范围。使用其他内标将使得定量丙二酸、二甲基丙二酸、乙基丙二酸、甲基琥珀酸、戊二酸和其他二羧酸成为可能。
