虎奶菇提取物（xLr®）在DAF-16抗氧化调节中对紫外线B辐射的保护作用。
Protective effects of tiger milk mushroom extract (xLr®) against UVB irradiation in DAF-16 anti-oxidant regulation.
作者信息
Rangsinth Panthakarn, Sharika Rajasekharan, Sillapachaiyaporn Chanin, Nilkhet Sunita, Chaikhong Kamonwan, Verma Kanika, Prasansuklab Anchalee, Ng Szu-Ting, Tan Chon-Seng, Fung Shin-Yee, Tencomnao Tewin, Chuchawankul Siriporn
机构信息
Immunomodulation of Natural Products Research Unit, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Transfusion Medicine and Clinical Microbiology, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
J Tradit Complement Med. 2024 Nov 12;15(1):73-83. doi: 10.1016/j.jtcme.2024.11.004. eCollection 2025 Jan.
BACKGROUND AND AIM
A critical causative factor of oxidative stress and inflammation leading to several skin complications is ultraviolet-B (UVB) irradiation. (LR), or tiger milk mushroom, is native to Southeast Asia. Cold water extract of an LR cultivar, TM02® (xLr®) is a promising anti-oxidant and anti-inflammatory source. However, the effects of xLr® on UVB-induced photoaging have never been elucidated.
EXPERIMENTAL PROCEDURE
This study investigated the protective effects of xLr® and its high, medium, and low molecular weight (HLR, MLR, and LLR, respectively) fractions against UVB irradiation using () model.
RESULTS AND CONCLUSION
The investigation revealed a significant lifespan extension of xLr® and its fractions in UVB-irradiated , which could be mediated by the regulation of genes associated with anti-oxidant ( and ) and apoptosis (, , , and ) pathways. xLr® significantly reduced the ROS production in and increased the DAF-16 nuclear translocation compared to untreated worms. Additionally, the SOD-3 expression was increased in the xLr®-treated worms. Hence, it suggests that the different components in xLr® work synergistically to protect against UVB irradiation. Our findings may be beneficial for the application of xLr® as a treatment against UVB-induced cellular damage and photoaging.
背景与目的
紫外线B（UVB）照射是导致多种皮肤并发症的氧化应激和炎症的关键致病因素。灵芝（LR），即虎奶菇，原产于东南亚。灵芝栽培品种TM02®（xLr®）的冷水提取物是一种很有前景的抗氧化和抗炎来源。然而，xLr®对UVB诱导的光老化的影响尚未阐明。
实验步骤
本研究使用秀丽隐杆线虫模型研究了xLr®及其高、中、低分子量（分别为HLR、MLR和LLR）组分对UVB照射的保护作用。
结果与结论
研究发现，xLr®及其组分可显著延长UVB照射的秀丽隐杆线虫的寿命，这可能是通过调节与抗氧化（sod-3和ctl-2）和凋亡（cep-1、ced-3、ced-4和ced-9）途径相关的基因来介导的。与未处理的线虫相比，xLr®显著降低了秀丽隐杆线虫体内的活性氧产生，并增加了DAF-16的核转位。此外，xLr®处理的线虫中SOD-3的表达增加。因此，这表明xLr®中的不同成分协同发挥作用以抵御UVB照射。我们的研究结果可能有助于将xLr®应用于治疗UVB诱导的细胞损伤和光老化。
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