Natural Products for Neuroprotection and Anti-Ageing (Neur-Age Natura) Research Unit, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Nutrients. 2022 Sep 5;14(17):3668. doi: 10.3390/nu14173668.
Hyperglycemia is one of the important causes of neurodegenerative disorders and aging. Pierre ex Lec (AC) has been widely used to relieve various health ailments. However, the neuroprotective and anti-aging effects against high glucose induction have not been investigated. This study aimed to investigate the effects of hexane extract of AC leaves (ACH) in vitro using human neuroblastoma SH-SY5Y cells and in vivo using nematode . SH-SY5Y cells and were pre-exposed with high glucose, followed by ACH treatment. To investigate neuroprotective activities, neurite outgrowth and cell cycle progression were determined in SH-SY5Y cells. In addition, was used to determine ACH effects on antioxidant activity, longevity, and healthspan. In addition, ACH phytochemicals were analyzed and the possible active compounds were identified using a molecular docking study. ACH exerted neuroprotective effects by inducing neurite outgrowth via upregulating growth-associated protein 43 and teneurin-4 expression and normalizing cell cycle progression through the regulation of cyclin D1 and SIRT1 expression. Furthermore, ACH prolonged lifespan, improved body size, body length, and brood size, and reduced intracellular ROS accumulation in high glucose-induced via the activation of gene expression in the DAF-16/FoxO pathway. Finally, phytochemicals of ACH were analyzed and revealed that β-sitosterol and stigmasterol were the possible active constituents in inhibiting insulin-like growth factor 1 receptor (IGFR). The results of this study establish ACH as an alternative medicine to defend against high glucose effects on neurotoxicity and aging.
高血糖是神经退行性疾病和衰老的重要原因之一。Pierre ex Lec（AC）已被广泛用于缓解各种健康疾病。然而，其对高糖诱导的神经保护和抗衰老作用尚未得到研究。本研究旨在通过体外培养的人神经母细胞瘤 SH-SY5Y 细胞和体内培养的秀丽隐杆线虫来研究 AC 叶的正己烷提取物（ACH）的作用。SH-SY5Y 细胞和 先用高葡萄糖孵育，然后用 ACH 处理。为了研究神经保护活性，在 SH-SY5Y 细胞中测定神经突生长和细胞周期进程。此外，使用 来确定 ACH 对抗氧化活性、寿命和健康跨度的影响。此外，通过分子对接研究分析了 ACH 中的植物化学物质，并鉴定了可能的活性化合物。ACH 通过上调生长相关蛋白 43 和 tenurin-4 的表达诱导神经突生长，通过调节细胞周期蛋白 D1 和 SIRT1 的表达使细胞周期进程正常化，从而发挥神经保护作用。此外，ACH 通过激活 DAF-16/FoxO 途径中的基因表达，延长了高葡萄糖诱导的 的寿命，改善了体型、体长和产卵量，并减少了细胞内 ROS 的积累。最后，分析了 ACH 的植物化学物质，并表明 β-谷甾醇和豆甾醇是抑制胰岛素样生长因子 1 受体（IGFR）的可能活性成分。本研究结果确立了 ACH 作为一种替代医学方法，可用于防御高血糖对神经毒性和衰老的影响。
