Therapeutic efficacy of against phenylhydrazine-induced hemolytic anemia in rats.

BACKGROUND & AIM: Hemolytic anemia is a blood disorder whose incidence is increasing in the world in recent years especially after the pandemic. Conventional treatments include use of steroids and immunosuppresants that are accompanied by numerous adverse effects. With growing interest in using complex multi-component formulations for multi-targeted therapy, the present study aims to investigate the therapeutic efficacy of a traditional herbomineral preparation, , which has been traditionally used as a supplement in iron-deficiency anemia, against phenylhydrazine-induced hemolytic anemia in rodent models.

EXPERIMENTAL APPROACHES

We employ a combination of and methods in this work to study the therapeutic potential and to understand the possible molecular targets of this traditional formulation. Conventional drugs prednisolone and ferrous sulphate were used for comparison.

RESULTS AND CONCLUSION

The studies confirm the ability of to reverse pathological changes associated with hemolytic anemia at 100 mg/kg and 200 mg/kg concentration. It restored hemoglobin, bilirubin and white blood cell levels to normal and reduced reticulocytes, hemosiderin and Gamna Gandy bodies in the liver, spleen and kidney. studies suggested that the key constituents in interact with high affinity to erythropoietic receptor which could contribute to erythropoiesis. The study also predicted that the phytoconstituents of could inhibit TNF-α activity which was validated using gene expression studies.