评估快速诊断试剂盒检测病毒性肠胃炎病原体的有效性。
Assessing the Effectiveness of Fast-Track Diagnostic Kit for Detecting Viral Gastroenteritis Agents.
作者信息
Pham Ngan T K, Hanaoka Nozomu, Trinh Quang D, Shimizu-Onda Yuko, Khamrin Pattara, Hoque Sheikh A, Komine-Aizawa Shihoko, Okitsu Shoko, Hayakawa Satoshi, Yoshimune Kazuaki, Ushijima Hiroshi
出版信息
Clin Lab. 2025 Jan 1;71(1). doi: 10.7754/Clin.Lab.2024.240821.
BACKGROUND
Viral gastroenteritis is a significant global health concern. An effective, rapid, and easy-to-use diagnostic tool is essential for screening causative viruses.
METHODS
Forty-eight samples, known to be infected with one of the following viruses: norovirus, group A rotavirus, astrovirus, adenovirus, and sapovirus determined by reverse transcription-PCR and nucleotide sequencing, were evaluated by the Fast Track Diagnostics (FTD) viral gastroenteritis assay.
RESULTS
The assay demonstrated 100% specificity for all viruses and matched the RT-PCR sensitivity for norovi-rus GI, classic human astrovirus, adenovirus, and sapovirus. It identified norovirus GII and rotavirus with 87.5% and 85.7% sensitivity, respectively. However, its sensitivity for detecting novel human astrovirus MLB and VA was lower, at 35%.
CONCLUSIONS
The FTD viral gastroenteritis assay can effectively screen simultaneously for norovirus GI, GII, group A rotavirus, adenovirus, and sapovirus in clinical settings. The study also suggests that improved detection methods are necessary for novel astrovirus strains.
背景
病毒性肠胃炎是一个重大的全球健康问题。一种有效、快速且易于使用的诊断工具对于筛查致病病毒至关重要。
方法
通过逆转录-聚合酶链反应和核苷酸测序确定的48份已知感染以下病毒之一的样本：诺如病毒、A组轮状病毒、星状病毒、腺病毒和札幌病毒，采用快速诊断（FTD）病毒性肠胃炎检测方法进行评估。
结果
该检测方法对所有病毒的特异性均为100%，并且与诺如病毒GI、经典人类星状病毒、腺病毒和札幌病毒的逆转录-聚合酶链反应敏感性相匹配。它分别以87.5%和85.7%的敏感性鉴定出诺如病毒GII和轮状病毒。然而，其检测新型人类星状病毒MLB和VA的敏感性较低，为35%。
结论
FTD病毒性肠胃炎检测方法可在临床环境中有效地同时筛查诺如病毒GI、GII、A组轮状病毒、腺病毒和札幌病毒。该研究还表明，对于新型星状病毒株，需要改进检测方法。
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