从伊拉克患者阴道和口腔念珠菌病分离出的念珠菌属某些毒力因子的检测
Detection of Some Virulence Factors from Candida spp. Isolated from Vaginal and Oral Candidiasis in Iraqi Patients.
作者信息
Maryoush Sara S, Ajah Hamzia A, Abdulrazaq Raghad A
出版信息
Clin Lab. 2025 Jan 1;71(1). doi: 10.7754/Clin.Lab.2024.240304.
BACKGROUND
Candidiasis can be present as a cutaneous, mucosal, or deep-seated organ infection, which is caused by more than 20 types of Candida spp., with C. albicans being the most common. Hence, this work aimed to estimate some virulence factors, including phospholipase and biofilm formation, in some Candida spp.
METHODS
A total of eighty-six specimens were collected from patients with oral and vaginal candidiasis and subjected into different examinations, including cultural characteristic (on Sabouraud Dextrose Agar SDA and chromogenic Candida agar) and microscopic examination and germ tube formation (GT)to isolate Candida spp. In addition, the egg-yolk agar plate method was used to determine the extracellular phospholipase production, and the microtiter plate method was used to determine biofilm formation of Candida spp. Vitek Compact equipment was used to identify the highest phospholipase and biofilm-producers of Candida spp.
RESULTS
As a result of all examinations, 58.1% (n = 50/86) of isolates of Candida spp. were obtained, including 26.7% (n = 23/86) isolates of Candida spp. from oral cavity and 31.3% (n = 27/86) isolates of Candida spp. from vaginal cavity. These isolates included 58% (n = 29/50) C. albicans, 10% (n = 5/50) C. glabrata, 6% (n = 3/50) C. parasilosis, 6% (n = 3/50) C. krusei, 6% (n = 3/50) C. lusitaniae, 6% (n = 3/50) C. kefyr, 6% (n = 3/50) C. tropicalis, and 2% (n = 1/50) C. ciferrii. To quantify extracellular phospholipase production, the egg-yolk agar plate method was utilized. The results indicated that the majority of isolates (n = 33; 66%) were phospholipase-strong producers, 18% (n = 9) of isolates were phospholipase-moderate producers, 5% (n = 10) were phospholipase-weak producers, and 6% (n = 3) were non-phospholipase producers. Microtiter plate method was utilized to estimate formation of biofilm by Candida spp. obtained from vaginal and oral cavities. The majority of Candida spp. isolates (n = 32; 64%) were biofilm-strong producers, followed by 30% (n=15) moderate-biofilm producers and 6% (n = 3) weak-biofilm producers. The results of VITEK 2 system indicated that the probability of C. albicans, C. krusei, C. kefyr, C. tropicalis, C. lusitaniae, C. glabrata, and C. ciferrii was 98, 95, 94, 91, 85, 93, and 85 %, respectively.
CONCLUSIONS
Candida albicans was the most frequent isolate among all isolates.
背景
念珠菌病可表现为皮肤、黏膜或深部器官感染，由20多种念珠菌属引起，其中白色念珠菌最为常见。因此，本研究旨在评估某些念珠菌属的一些毒力因子，包括磷脂酶和生物膜形成。
方法
从口腔和阴道念珠菌病患者中总共收集了86份标本，并进行了不同的检查，包括培养特性（在沙氏葡萄糖琼脂培养基和显色念珠菌琼脂上）、显微镜检查和芽管形成试验（GT）以分离念珠菌属。此外，采用蛋黄琼脂平板法测定细胞外磷脂酶的产生，采用微量滴定板法测定念珠菌属的生物膜形成。使用Vitek Compact设备鉴定念珠菌属中磷脂酶和生物膜产生能力最强的菌株。
结果
经过所有检查，共获得58.1%（n = 50/86）的念珠菌属分离株，其中26.7%（n = 23/86）的念珠菌属分离株来自口腔，31.3%（n = 27/86）的念珠菌属分离株来自阴道。这些分离株包括58%（n = 29/50）的白色念珠菌、10%（n = 5/50）的光滑念珠菌、6%（n = 3/50）的近平滑念珠菌、6%（n = 3/50）的克柔念珠菌、6%（n = 3/50）的葡萄牙念珠菌、6%（n = 3/50）的凯菲念珠菌、6%（n = 3/50）的热带念珠菌和2%（n = 1/50）的西弗念珠菌。为了量化细胞外磷脂酶的产生，采用了蛋黄琼脂平板法。结果表明，大多数分离株（n = 33；66%）是磷脂酶强产生者，18%（n = 9）的分离株是磷脂酶中度产生者，5%（n = 10）是磷脂酶弱产生者，6%（n = 3）是非磷脂酶产生者。采用微量滴定板法评估从阴道和口腔获得的念珠菌属生物膜的形成。大多数念珠菌属分离株（n = 32；64%）是生物膜强产生者，其次是30%（n = 15）的中度生物膜产生者和6%（n = 3）的弱生物膜产生者。VITEK 2系统的结果表明，白色念珠菌、克柔念珠菌、凯菲念珠菌、热带念珠菌、葡萄牙念珠菌、光滑念珠菌和西弗念珠菌的鉴定概率分别为98%、95%、94%、91%、85%、93%和85%。
结论
白色念珠菌是所有分离株中最常见的菌株。
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