Kantarcioglu A Serda, Yücel A
Department of Microbiology and Clinical Microbiology, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul Turkey.
Mycoses. 2002 Jun;45(5-6):160-5. doi: 10.1046/j.1439-0507.2002.00727.x.
The present study was aimed at determining in vitro phospholipase and protease activities in 95 clinical isolates of various Candida species (C. albicans, C. glabrata, C. guilliermondii,C.kefyr, C. krusei, C. lipolytica, C. lusitaniae, C. parapsilosis, C.rugosa and C. tropicalis). 59 (62.1%) of isolates examined were phospholipase positive and 75 (78.9.9%) were protease positive. 56 (93.3%) of C.albicans isolates tested were phospholipase producers - however only a few strains of C. glabrata and C. kefyr behaved in the same way. Protease activity was detected in 57 (95%) of C. albicans strains tested and in a few strains of C. kefyr, C.lipolytica, C. parapsilosis and C. tropicalis- the remaining isolates were negative. 56 strains of C.albicans and 2 strains of C. kefyr tested produced both phospholipase and protease.
本研究旨在测定95株不同念珠菌属临床分离株（白色念珠菌、光滑念珠菌、季也蒙念珠菌、克柔念珠菌、克鲁斯念珠菌、解脂念珠菌、葡萄牙念珠菌、近平滑念珠菌、皱褶念珠菌和热带念珠菌）的体外磷脂酶和蛋白酶活性。检测的分离株中有59株（62.1%）磷脂酶呈阳性，75株（78.9%）蛋白酶呈阳性。检测的白色念珠菌分离株中有56株（93.3%）产生磷脂酶——然而只有少数光滑念珠菌和克柔念珠菌菌株有同样的表现。在检测的57株（95%）白色念珠菌菌株以及少数克柔念珠菌、解脂念珠菌、近平滑念珠菌和热带念珠菌菌株中检测到蛋白酶活性——其余分离株为阴性。检测的56株白色念珠菌和2株克柔念珠菌产生磷脂酶和蛋白酶。
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