Shi Jinxing, Kong Zhongxin, Zhong Jinkun, Zhang Xiaoqiu, Luo Xianmin, Wang Heyu, Xu Boyang, Wang Xin, Cheng Rui, Yuan Yang, Li Na, Xie Quan, Li Guoqiang, Jia Haiyan, Ma Zhengqiang
The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre and State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, China.
Zhongshan Biological Breeding Laboratory, Nanjing, China.
Nat Genet. 2025 Feb;57(2):310-313. doi: 10.1038/s41588-024-02056-3. Epub 2025 Jan 14.
Ambiguity about whether the histidine-rich calcium-binding protein-coding gene (His) or the pore-forming toxin-like gene (PFT) or both are responsible for Fusarium head blight (FHB) resistance conferred by the Fhb1 quantitative trait locus hinders progress toward elucidating Fhb1 resistance mechanisms. Here, with a series of developed lines with or without PFT but all possessing His and five His-carrying PFT mutant lines created via gene editing, we show that PFT does not confer FHB resistance and that the His resistance effect does not require PFT in the tested conditions. We also show that PFT mutations are not associated with morphological and phenological characteristics that often affect FHB severity.
富含组氨酸的钙结合蛋白编码基因（His）、成孔毒素样基因（PFT）或两者是否对由Fhb1数量性状基因座赋予的赤霉病（FHB）抗性负责尚不清楚，这阻碍了阐明Fhb1抗性机制的研究进展。在此，我们利用一系列具有或不具有PFT但均含有His的育成系，以及通过基因编辑创建的五个携带His的PFT突变系，表明PFT不赋予FHB抗性，并且在测试条件下His的抗性效应不需要PFT。我们还表明，PFT突变与通常影响FHB严重程度的形态和物候特征无关。
