Carril Pablo, Cordeiro Carlos, Silva Marta Sousa, Ngendahimana Ephrem, Tenreiro Rogério, Cruz Cristina
Plant-Soil Ecology Laboratory, Center for Ecology, Evolution and Environmental Changes. Faculty of Sciences, University of Lisbon, Lisbon, Portugal.
Department of Biology, Università Degli Studi Di Firenze, Via Micheli 1, 50121, Florence, Italy.
Planta. 2025 Jan 14;261(2):36. doi: 10.1007/s00425-025-04609-0.
Inoculation with the PGPB Herbaspirillum seropedicae shapes both the structure and putative functions of the wheat microbiome and causes changes in the levels of various plant metabolites described to be involved in plant growth and health. Plant growth promoting bacteria (PGPB) can establish metabolic imprints in their hosts, contributing to the improvement of plant health in different ways. However, while PGPB imprints on plant metabolism have been extensively characterized, much less is known regarding those affecting plant indigenous microbiomes, and hence it remains unknown whether both processes occur simultaneously. In this study, both 16S amplicon and ITS sequencing analyses were carried out to study both the structural as well as the putative functional changes in the seed-borne endophytic microbiome of wheat plants inoculated with the PGPB Herbaspirillum seropedicae strain RAM10. Concomitantly, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) analyses were used to investigate the alterations in the root metabolome of PGPB-inoculated plants. PGPB inoculation led to marked differences in the composition of the root microbiome, accompanied by the differential enrichment of microorganisms with putative roles in both plant energy and nitrogen metabolism. In addition, metabolome analyses showed that the levels of 16 metabolites belonging to the phenylpropanoid, terpenoid, and unsaturated fatty acid families were significantly altered in PGPB-inoculated plants. These findings shed light on the interplay between PGPB, the plant and its associated microbiome, indicating that PGPB can act as the driving force mediating long-lasting changes in both the plant metabolome and the plant microbiome.
接种植物促生细菌（PGPB）巴西固氮螺菌会塑造小麦微生物组的结构和假定功能，并导致各种参与植物生长和健康的植物代谢物水平发生变化。植物促生细菌可以在其宿主中建立代谢印记，以不同方式促进植物健康。然而，虽然PGPB对植物代谢的印记已得到广泛表征，但对影响植物原生微生物组的印记了解较少，因此这两个过程是否同时发生仍不清楚。在本研究中，进行了16S扩增子和ITS测序分析，以研究接种PGPB巴西固氮螺菌菌株RAM10的小麦植株种子携带的内生微生物组的结构和假定功能变化。同时，利用傅里叶变换离子回旋共振质谱（FT-ICR-MS）分析来研究接种PGPB的植物根系代谢组的变化。接种PGPB导致根系微生物组组成存在显著差异，同时在植物能量和氮代谢中具有假定作用的微生物有不同程度的富集。此外，代谢组分析表明，接种PGPB的植物中属于苯丙烷类、萜类和不饱和脂肪酸家族的16种代谢物水平发生了显著变化。这些发现揭示了PGPB、植物及其相关微生物组之间的相互作用，表明PGPB可以作为介导植物代谢组和植物微生物组长期变化的驱动力。
