Azami Mohammad Masrin, Pauzi Nur Aimi Syarina, Hamdan Noazlina, Sidik Muhammad Redzwan, Tulis Aisya Naama, Hassan Roslina, Saeid Faizah Hanim Mohd
Veterinary Research Institute (VRI), 59 Jalan Sultan Azlan Shah, 31400, Ipoh, Perak, Malaysia.
Vet Res Commun. 2025 Jan 15;49(2):77. doi: 10.1007/s11259-025-10645-8.
African swine fever (ASF), a severe and highly contagious haemorrhagic viral disease of pigs, is becoming a major threat not only in Malaysia but around the world. The first confirmed case of ASF in Malaysia was reported in February 2021. Despite the emergence of ASF in Malaysia, genetic information on this causative pathogen for the local livestock is still limited. This study aimed to genetically characterize the African swine fever virus (ASFV) responsible for the 2021 outbreak in Malaysia. The genome of the ASFV isolated during the first outbreak in Malaysia was analysed as ASFV/Sabah/Malaysia/1160/21 which has 190,594 base pairs, with a nitrogenous bases (GC) content of 40.33% and 195 predicted Open Reading Frames (ORF). The complete genome sequence was compared with other annotated ASFV genomes retrieved from database of National Center for Biotechnology Information (NCBI) to obtain information based on target gene B646L, E183L, intergenic region (IGR) between I73R and I329L (IGR), EP402R and B602L. The ASFV/Sabah/Malaysia/1160/21 genome had a high similarity percentage to the reference genome, Georgia 2007 and all Southeast Asian strains. Phylogenetic analysis revealed that the ASFV strain belonged to genotype II, serogroup 8, CVR1 and showed high characteristics of IGR variant II based on IGR. This study expands our understanding of genetic diversity and provides significant insights into the genomic characteristics and variation of ASFV strains that are circulating in Malaysia.
非洲猪瘟（ASF）是一种严重且极具传染性的猪出血性病毒性疾病，不仅在马来西亚，而且在全球范围内都正成为一个重大威胁。马来西亚于2021年2月报告了首例ASF确诊病例。尽管ASF在马来西亚出现，但关于这种导致当地牲畜发病的病原体的基因信息仍然有限。本研究旨在对导致2021年马来西亚疫情爆发的非洲猪瘟病毒（ASFV）进行基因特征分析。对在马来西亚首次疫情爆发期间分离出的ASFV基因组进行了分析，该基因组被命名为ASFV/沙巴/马来西亚/1160/21，有190,594个碱基对，含氮碱基（GC）含量为40.33%，有195个预测的开放阅读框（ORF）。将完整的基因组序列与从美国国立生物技术信息中心（NCBI）数据库中检索到的其他注释过的ASFV基因组进行比较，以获取基于目标基因B646L、E183L、I73R和I329L之间的基因间隔区（IGR）、EP402R和B602L的信息。ASFV/沙巴/马来西亚/1160/21基因组与参考基因组格鲁吉亚2007以及所有东南亚毒株具有很高的相似百分比。系统发育分析表明，该ASFV毒株属于II基因型、血清群8、CVR1，并且基于IGR显示出IGR变体II的高度特征。本研究扩展了我们对基因多样性的理解，并为在马来西亚传播的ASFV毒株的基因组特征和变异提供了重要见解。
