Silva Lucas Felipe, Barreto Kellya F M, Silva Helaine Cristiane, de Souza Iara Dantas, Meneses Carlos H S G, Uchôa Adriana F, Scortecci Katia C
Bioinformatics Multidisciplinary Environment, IMD, Universidade Federal Do Rio Grande Do Norte, Natal, Brazil.
LPTAM, Universidade Federal Do Rio Grande Do Norte, Natal, Brazil.
BMC Plant Biol. 2025 Jan 14;25(1):54. doi: 10.1186/s12870-024-06036-x.
Significant changes in the proteome highlight essential metabolic adaptations for development and oxidative signaling induced by the treatment of young sugarcane plants with hydrogen peroxide. These adaptations suggest that hydrogen peroxide acts not only as a stressor but primarily as a signaling molecule, triggering specific metabolic pathways that regulate growth and plant resilience. Sugarcane is a crucial crop for sugar and ethanol production, often influenced by environmental signals. Hydrogen peroxide (HO) is increasingly recognized as an important signaling molecule that regulates plant development and adaptation. In this study, two-month-old sugarcane plants were treated with varying concentrations of HO to investigate how this molecule acts as a signal at the cellular, biochemical, and proteomic levels. Antioxidant enzyme activity exhibited fluctuations, suggesting a dynamic response to oxidative signaling. Lipid peroxidation, observed through TBARs and scanning electron microscopy, highlighted early membrane modifications. Proteomic analysis (ProteomeXchange PXD048142) identified 2,699 proteins, with 155 showing significant expression changes in response to HO signaling. Bioinformatics, including Principal Component Analysis, revealed distinct proteomic profiles in roots and leaves, indicating tissue-specific metabolic reprogramming. Functional annotation through Gene Ontology and KEGG pathway enrichment showed that oxidative signaling led to the repression of photosynthesis-related pathways in leaves, while promoting pathways related to protein processing, glycolysis, and carbon metabolism in roots. Additionally, bioinformatic tools identified proteins involved in amino acid metabolism, the TCA cycle, and carbohydrate metabolism as critical components of sugarcane's adaptive signaling response. The data suggest that sugarcane plants responded to oxidative signals by adjusting their metabolic networks, promoting sustained development and potential pathways for targeted plant breeding.
蛋白质组的显著变化突出了过氧化氢处理幼嫩甘蔗植株所诱导的发育和氧化信号传导过程中至关重要的代谢适应性。这些适应性表明，过氧化氢不仅作为一种应激源，而且主要作为一种信号分子，触发调节生长和植物抗性的特定代谢途径。甘蔗是糖和乙醇生产的关键作物，常受环境信号影响。过氧化氢（H₂O₂）越来越被认为是调节植物发育和适应性的重要信号分子。在本研究中, 用不同浓度的H₂O₂处理两个月大的甘蔗植株，以研究该分子在细胞、生化和蛋白质组水平上作为信号的作用方式。抗氧化酶活性呈现波动，表明对氧化信号有动态响应。通过硫代巴比妥酸反应物（TBARs）和扫描电子显微镜观察到的脂质过氧化作用突出了早期膜修饰。蛋白质组分析（ProteomeXchange PXD048142）鉴定出2699种蛋白质，其中155种蛋白质在响应H₂O₂信号时表现出显著的表达变化。包括主成分分析在内的生物信息学揭示了根和叶中不同的蛋白质组图谱，表明存在组织特异性的代谢重编程。通过基因本体论和KEGG通路富集进行的功能注释表明，氧化信号导致叶片中光合作用相关通路的抑制，而促进根中与蛋白质加工、糖酵解和碳代谢相关的通路。此外，生物信息学工具确定参与氨基酸代谢、三羧酸循环和碳水化合物代谢的蛋白质是甘蔗适应性信号反应的关键组成部分。数据表明，甘蔗植株通过调整其代谢网络对氧化信号作出反应，促进持续发育以及为定向植物育种提供潜在途径。
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