Sehic Edina, de Miguel-Gómez Lucía
Department of Medical Cell Biology, Uppsala University, 75123 Uppsala, Sweden.
Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, Kvinnokliniken, Blå stråket 6, 40530 Gothenburg, Sweden.
Regen Ther. 2024 Dec 21;28:183-190. doi: 10.1016/j.reth.2024.12.011. eCollection 2025 Mar.
Bioengineering is applied in different areas, including women's infertility management. Among other approaches, decellularized tissues are being used to treat uterine disorders causing infertility. Biomaterials made from decellularized tissue consist of tissue-specific extracellular matrix and, as acellular scaffolds, are thought to be immune inert. Hence, they are good grafting candidates to replace and regenerate excised damaged uterine tissue to cure infertility. However, decellularization approaches differ among species and research groups, posing challenges for comparison and standardization. The diversity in data reporting and studied properties of the resulting decellularized scaffold make it even more difficult, especially when the ultimate goal is clinical translation. Thus, this review aims to critically assess whole uterus decellularization studies, extracting and comparing their main results and conclusions. After carefully evaluating the reviewed studies, we noticed that the vast majority base the uterus decellularization success and resulting scaffold efficacy on the DNA removal efficacy, while other crucial aspects, including the extracellular matrix integrity or immunogenicity, are underestimated. Thus, this review further proposes practical points for what should be considered and how results can be reported in studies involving whole uterus decellularization to facilitate comparison between studies and translational progress.
生物工程应用于不同领域，包括女性不孕症的治疗。在其他方法中，脱细胞组织正被用于治疗导致不孕的子宫疾病。由脱细胞组织制成的生物材料由组织特异性细胞外基质组成，作为无细胞支架，被认为具有免疫惰性。因此，它们是很好的移植候选物，可用于替代和再生切除的受损子宫组织以治疗不孕症。然而，脱细胞方法在不同物种和研究组之间存在差异，这给比较和标准化带来了挑战。数据报告的多样性以及所产生的脱细胞支架的研究特性使得这一情况更加困难，尤其是当最终目标是临床转化时。因此，本综述旨在批判性地评估全子宫脱细胞研究，提取并比较其主要结果和结论。在仔细评估所综述的研究后，我们注意到绝大多数研究将子宫脱细胞的成功与否以及所得支架的功效基于DNA去除功效，而其他关键方面，包括细胞外基质完整性或免疫原性，则被低估。因此，本综述进一步提出了一些实用要点，即在涉及全子宫脱细胞的研究中应考虑哪些因素以及如何报告结果，以促进研究之间的比较和转化进展。
