Stoian Alina, Adil Aisha, Biniazan Felor, Haykal Siba
Latner Thoracic Research Laboratories, Division of Thoracic Surgery, Toronto General Hospital Research Institute, University Health Network, Toronto, ON M5G 1L7, Canada.
Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON M5S 1A8, Canada.
Curr Issues Mol Biol. 2024 Aug 22;46(8):9179-9214. doi: 10.3390/cimb46080543.
The recellularization of tissues after decellularization is a relatively new technology in the field of tissue engineering (TE). Decellularization involves removing cells from a tissue or organ, leaving only the extracellular matrix (ECM). This can then be recellularized with new cells to create functional tissues or organs. The first significant mention of recellularization in decellularized tissues can be traced to research conducted in the early 2000s. One of the landmark studies in this field was published in 2008 by Ott, where researchers demonstrated the recellularization of a decellularized rat heart with cardiac cells, resulting in a functional organ capable of contraction. Since then, other important studies have been published. These studies paved the way for the widespread application of recellularization in TE, demonstrating the potential of decellularized ECM to serve as a scaffold for regenerating functional tissues. Thus, although the concept of recellularization was initially explored in previous decades, these studies from the 2000s marked a major turning point in the development and practical application of the technology for the recellularization of decellularized tissues. The article reviews the historical advances and limitations in organ recellularization in TE over the last two decades.
组织脱细胞后再细胞化是组织工程（TE）领域中一项相对较新的技术。脱细胞过程涉及从组织或器官中去除细胞，仅留下细胞外基质（ECM）。然后可以用新细胞对其进行再细胞化，以创建功能性组织或器官。脱细胞组织中首次有意义地提及再细胞化可追溯到21世纪初进行的研究。该领域的一项具有里程碑意义的研究于2008年由奥特发表，研究人员在其中展示了用心脏细胞对脱细胞大鼠心脏进行再细胞化，从而得到一个能够收缩的功能性器官。从那时起，其他重要研究也相继发表。这些研究为再细胞化在TE中的广泛应用铺平了道路，证明了脱细胞ECM作为再生功能性组织支架的潜力。因此，尽管再细胞化的概念在之前几十年就已初步探索，但21世纪的这些研究标志着脱细胞组织再细胞化技术发展和实际应用中的一个重大转折点。本文回顾了过去二十年中TE领域器官再细胞化的历史进展和局限性。
