Zhu M, Guo T, Liu Y B, Xiao R, Yu T, Huang J X, Du W L, Zhong X M, Song B, Li F H
College of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, 110866 Shenyang, Liaoning Province, China.
Affiliated Experimental Field, Shenyang Agricultural University, 110866 Shenyang, Liaoning Province, China.
Photosynthetica. 2024 Dec 3;62(4):361-371. doi: 10.32615/ps.2024.039. eCollection 2024.
Melatonin (MT), an indole compound, can boost plant growth under abiotic stress conditions. This experiment aims to elucidate the synergistic effect of MT and ascorbic acid (AsA) in mitigating salinity stress by assessing the photosynthetic and antioxidant capacity of the maize inbred lines H123 and W961. The results indicated that exogenous MT and AsA significantly improved photosynthetic efficiency and biomass of maize under salinity stress. Additionally, exogenous MT and AsA also improved antioxidant enzyme activities, promoted regeneration of AsA and GSH, decreased reactive oxygen species contents, suppressed Na accumulation, and improved the K/Na ratio of maize seedlings. Additionally, the AsA inhibitor lycorine decreased the endogenous content of AsA and eliminated the positive effects of MT, while the MT inhibitor chlorophenyl alanine (CPA) reduced the endogenous content of MT, which could not eliminate the promoting effects of AsA. The results suggested that AsA may act as a downstream signal involved in the regulatory effects of MT on maize under salinity stress.
褪黑素（MT）是一种吲哚化合物，在非生物胁迫条件下能够促进植物生长。本实验旨在通过评估玉米自交系H123和W961的光合及抗氧化能力，阐明MT与抗坏血酸（AsA）在缓解盐胁迫方面的协同作用。结果表明，外源MT和AsA显著提高了盐胁迫下玉米的光合效率和生物量。此外，外源MT和AsA还提高了抗氧化酶活性，促进了AsA和谷胱甘肽（GSH）的再生，降低了活性氧含量，抑制了Na积累，并提高了玉米幼苗的K/Na比值。此外，AsA抑制剂石蒜碱降低了AsA的内源含量并消除了MT的积极作用，而MT抑制剂对氯苯丙氨酸（CPA）降低了MT的内源含量，但无法消除AsA的促进作用。结果表明，AsA可能作为下游信号参与MT在盐胁迫下对玉米的调控作用。
