Liu Yingda, Pu Yu, Kuang Xunjie, Jiang Yuzhu, Liu Jiali, Dai Nan, Li Mengxia, Li Kunkun, Xu Mingfang
Department of Cancer Center, Daping Hospital, Army Medical University, Chongqing, China.
Department of Thoracic Surgery, Daping Hospital, Army Medical University, Chongqing, China.
J Gastrointest Oncol. 2024 Dec 31;15(6):2400-2412. doi: 10.21037/jgo-24-331. Epub 2024 Dec 18.
Esophageal squamous cell carcinoma (ESCC) is a highly prevalent and malignant form of esophageal tumor associated with high rates of patient mortality for which there remains a persistent lack of effective targets for therapeutic interventional efforts. This study was developed with the goal of exploring the expression and functional role of RBM15 in ESCC.
This study was developed with the goal of exploring the expression and functional role of RBM15 in ESCC. To establish the prognostic and therapeutic significance of RBM15 in this cancer, data from the Gene Expression Omnibus (GEO), The Cancer Genome Atlas (TCGA), and UCSC Xena databases were leveraged. Immunohistochemical analyses were used to assess RBM15 expression in postoperative ESCC tumor tissue samples, and the correlations between such expression and patient outcomes were assessed. The effects of RBM15 on ESCC cell proliferative, migratory, and invasive activity were assessed with Cell Counting Kit-8 (CCK8) and Transwell assay approaches. Together, these experiments revealed RBM15 upregulation in ESCC relative to paracancerous tissues, while confirming that it was associated with favorable patient outcomes. RBM15 was also found to suppress ESCC cell proliferation, migration, and invasivity.
We suggest that RBM15 may be a clinically relevant prognostic factor in ESCC such that new therapeutic interventions based on low levels of RBM15 have the potential to be developed for the improved management of ESCC in the future.
The results of the present study provide confirmation that high levels of RBM15 expression are protective and associated with better ESCC patient prognostic outcomes. Pan-cancer analyses performed herein also revealed the correlations between RBM15 expression and prognosis in various cancers.
食管鳞状细胞癌（ESCC）是一种高度普遍且恶性的食管肿瘤形式，患者死亡率很高，目前仍持续缺乏有效的治疗干预靶点。本研究旨在探索RBM15在ESCC中的表达及功能作用。
本研究旨在探索RBM15在ESCC中的表达及功能作用。为确定RBM15在该癌症中的预后和治疗意义，利用了基因表达综合数据库（GEO）、癌症基因组图谱（TCGA）和加州大学圣克鲁兹分校（UCSC）Xena数据库的数据。采用免疫组织化学分析评估RBM15在ESCC术后肿瘤组织样本中的表达，并评估这种表达与患者预后之间的相关性。用细胞计数试剂盒-8（CCK8）和Transwell分析法评估RBM15对ESCC细胞增殖、迁移和侵袭活性的影响。这些实验共同揭示了ESCC中RBM15相对于癌旁组织上调，同时证实其与患者良好预后相关。还发现RBM15可抑制ESCC细胞增殖、迁移和侵袭性。
我们认为RBM15可能是ESCC中一个具有临床相关性的预后因素，因此基于低水平RBM15的新治疗干预措施未来有可能被开发出来，用于改善ESCC的管理。
本研究结果证实RBM15高表达具有保护作用，并与ESCC患者更好的预后结果相关。本文进行的泛癌分析还揭示了RBM15表达与各种癌症预后之间的相关性。
