Bonar L C, Lees S, Mook H A
J Mol Biol. 1985 Jan 20;181(2):265-70. doi: 10.1016/0022-2836(85)90090-7.
Neutron diffraction measurements have been made of the equatorial and meridional spacings of collagen in fully mineralized mature bovine bone and demineralized bone collagen, in both wet and dry conditions. The collagen equatorial spacing in wet mineralized bovine bone is 1.24 nm, substantially lower than the 1.53 nm value observed in wet demineralized bovine bone collagen. Corresponding spacings for dry bone and demineralized bone collagen are 1.16 nm and 1.12 nm, respectively. The collagen meridional long spacing in mineralized bovine bone is 63.6 nm wet and 63.4 nm dry. These data indicate that collagen in fully mineralized bovine bone is considerably more closely packed than had been assumed previously, with a packing density similar to that of the relatively crystalline collagens such as wet rat tail tendon. The data also suggest that less space is available for mineral within the collagen fibrils in bovine bone than had previously been assumed, and that the major portion of the mineral in this bone must be located outside the fibrils.
已对完全矿化的成熟牛骨和脱矿骨胶原在湿态和干态下的赤道间距和子午线间距进行了中子衍射测量。湿态矿化牛骨中胶原的赤道间距为1.24纳米，大大低于湿态脱矿牛骨胶原中观察到的1.53纳米的值。干骨和脱矿骨胶原的相应间距分别为1.16纳米和1.12纳米。矿化牛骨中胶原的子午线长间距在湿态下为63.6纳米，在干态下为63.4纳米。这些数据表明，完全矿化牛骨中的胶原堆积比以前假设的要紧密得多，其堆积密度与相对结晶的胶原（如湿态大鼠尾腱）相似。数据还表明，牛骨胶原纤维内可供矿物质占据的空间比以前假设的要少，并且该骨中大部分矿物质必定位于纤维之外。
