Vasundaradevi R, Sarvajith M, Divyashree S, Deepa N, Achar Premila N, Sreenivasa M Y
Molecular Mycotoxicology Lab, Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru, 570 006, India.
WDRC, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia.
Sci Rep. 2025 Jan 16;15(1):2144. doi: 10.1038/s41598-025-85190-0.
Fifty-five lactic acid bacteria (LAB) were isolated from seven selected tropical fruits, with Solanum nigrum exhibiting the highest LAB prevalence and Couroupita guianenis and Musa fruits showing the lowest counts. Two strains isolated from Ficus racemosa demonstrated significant antifungal activity against Fusarium oxysporum. 16S rDNA sequencing identified these strains as Lactiplantibacillus plantarum MYSVCF3 and Lpb. argentoratensis MYSVCF5. The isolates displayed adaptability to a broad range of environmental conditions, including temperatures of 10-45 °C, pH 2-6, and salt up to 7%. The strains tolerated simulated gastrointestinal conditions of acid (pH-2), phenol (0.6%), and bile (0.3%) suggesting potential probiotic attributes. Lpb. argentoratensis MYSVCF5 inhibited F. oxysporum, two ESKAPE group bacteria (P. aeruginosa, S. aureus) plus S. paratyphi and E. coli. The cell-free supernatant (CFS) of Lpb. argentoratensis MYSVCF5 reduced the growth of fungal biomass by 94% and completely inhibited conidial germination, retaining activity even after extended cold storage. LC-MS/MS analysis identified organic acids in the CFS, with citric acid as the most abundant at 34.9 (± 0.3) µg/mL, followed by lactic (8.3 µg/mL) and malic acids (5.2 µg/mL). This study isolated a novel LAB, a potential candidate having probiotics and antifungal properties for application in food and agriculture.
从七种选定的热带水果中分离出55株乳酸菌，其中龙葵的乳酸菌患病率最高，炮弹树和香蕉的乳酸菌数量最少。从无花果中分离出的两株菌株对尖孢镰刀菌表现出显著的抗真菌活性。16S rDNA测序将这些菌株鉴定为植物乳杆菌MYSVCF3和阿让托拉乳杆菌MYSVCF5。这些分离株对广泛的环境条件具有适应性，包括10-45°C的温度、pH值2-6以及高达7%的盐浓度。这些菌株能够耐受模拟的胃肠道条件，如酸性（pH-2）、苯酚（0.6%）和胆汁（0.3%），表明它们具有潜在的益生菌特性。阿让托拉乳杆菌MYSVCF5对尖孢镰刀菌、两种ESKAPE组细菌（铜绿假单胞菌、金黄色葡萄球菌）以及副伤寒沙门氏菌和大肠杆菌具有抑制作用。阿让托拉乳杆菌MYSVCF5的无细胞上清液（CFS）使真菌生物量的生长减少了94%，并完全抑制了分生孢子萌发，即使在长时间冷藏后仍保持活性。LC-MS/MS分析确定了CFS中的有机酸，其中柠檬酸含量最高，为34.9（±0.3）μg/mL，其次是乳酸（8.3μg/mL）和苹果酸（5.2μg/mL）。本研究分离出一种新型乳酸菌，它是一种具有益生菌和抗真菌特性的潜在候选菌株，可应用于食品和农业领域。
