Ohkohchi N, Kasai M, Ohi R, Igarashi Y
Nihon Geka Gakkai Zasshi. 1985 Mar;86(3):290-7.
Presently there is no effective method for evaluating the intestinal absorption of sugar, amino acid and small peptide. Since the transmural potential difference (delta PD) is generated immediately when sugar, amino acid or small peptide are absorbed in the small intestine, we tried to utilize this potential difference for the evaluation of absorptive faculty. The delta PD is the product of influx and electrical transmural resistance. To evaluate the intestinal absorptive faculty by the delta PD it is necessary that the electrical resistance of the small intestine does not change throughout the life time and that sugars, amino acids and peptides are transported actively. In guinea pig, the electrical resistance of the small intestine was examined in postnatal development in vitro. Transport systems of sugars amino acid and peptide were also investigated. Through the entire life the electrical resistance of the small intestine remained at almost the same value and sugars and amino acid were completely transported actively from the birth. But small peptide was transported actively after the period of weanling. Evaluation of intestinal aborptive faculty of sugars, amino acids and small peptides is considered to be possible by the transmural potential difference.
目前尚无评估糖、氨基酸和小肽肠道吸收的有效方法。由于糖、氨基酸或小肽在小肠吸收时会立即产生跨膜电位差（δPD），我们试图利用这种电位差来评估吸收能力。δPD是内流和跨膜电阻的乘积。要通过δPD评估肠道吸收能力，小肠的电阻在整个生命周期内不能改变，并且糖、氨基酸和肽必须主动转运。在豚鼠中，体外研究了小肠电阻在出生后发育过程中的变化。还研究了糖、氨基酸和肽的转运系统。在整个生命周期中，小肠电阻几乎保持在相同的值，糖和氨基酸从出生起就完全通过主动转运。但小肽在断奶期后才开始主动转运。通过跨膜电位差评估糖、氨基酸和小肽的肠道吸收能力被认为是可行的。
