Ohkohchi N, Kasai M, Ohi R, Igarashi Y, Naganuma H
Nihon Geka Gakkai Zasshi. 1985 Dec;86(12):1590-5.
Since there was no effective method for evaluating the absorptive capacity in the small intestine, we devised a test for evaluating the absorptive capacity with potential difference. Potential difference is provided by electrical resistance of intestine and flux of substances. Previously, we reported that the electrical resistance of the small intestine in the guinea pigs had changed very slightly throughout the entire life, and that sugars and neutral amino acids have been transported completely activity from the birth. In addition, potential difference of glycyl-glycine reflected the uptake of the intestine after the period of weanling. We experimentally studied the electrical transmural resistance and absorptive capacity of the small intestine with various damages to the small intestine by 5-Fu, ischemia and long fasting. Histologically, swelling of nucleus, intracellular edema, dilatation of capillary vein, dropping of epithelial cells, etc., were seen in these models. But the electrical resistance was slightly changed in 10% of the cases. Potential differences by sugars or neutral amino acid ingestion accurately reflected their real flux. These facts suggest that the potential differences deficiently reflect the uptake of sugars and amino acids in the small intestine under conditions with malabsorption.
由于没有评估小肠吸收能力的有效方法，我们设计了一种利用电位差评估吸收能力的测试方法。电位差由肠电阻和物质通量提供。此前，我们报道过豚鼠小肠的电阻在其整个生命过程中变化非常小，并且糖类和中性氨基酸从出生起就已经完全活跃地被转运。此外，甘氨酰甘氨酸的电位差反映了断奶期后小肠的摄取情况。我们通过实验研究了5-氟尿嘧啶、缺血和长期禁食对小肠造成各种损伤后的小肠跨壁电阻和吸收能力。组织学上，在这些模型中可见细胞核肿胀、细胞内水肿、毛细血管静脉扩张、上皮细胞脱落等情况。但在10%的病例中电阻仅有轻微变化。摄入糖类或中性氨基酸后的电位差准确反映了它们的实际通量。这些事实表明，在吸收不良的情况下，电位差不能充分反映小肠中糖类和氨基酸的摄取情况。
