Khalid Aleena, Gill Maria Mushtaq, Khan Mahvish Aftab, Naqvi Sakeenah Hussain, Roshan Muhammad, Imtiaz Anam
Department of Microbiology, Armed Forces Institute of Pathology / National University of Medical Sciences, Rawalpindi, Pakistan.
Department of Dermatology, Pakistan Institute of Medical Sciences, Islamabad, Pakistan.
J Coll Physicians Surg Pak. 2025 Jan;35(1):44-48. doi: 10.29271/jcpsp.2025.01.44.
To evaluate Chicago Sky Blue (CSB) stain, Calcofluor white (CW) stain, and Potassium Hydroxide (KOH) mount for rapid diagnosis of dermatomycosis, using fungal culture as the gold standard.
Cross-sectional analytical study. Place and Duration of the Study: This study was conducted in the Department of Microbiology, Armed Forces Institute of Pathology / National University of Medical Sciences, Rawalpindi, Pakistan, from July 2023 to February 2024.
Clinical specimens were collected from patients suspected of having dermatomycosis, including skin scrapings, hair, and nails. Each sample was divided into two parts. The first part underwent three microscopic techniques, namely 10% KOH mount, CSB stain, and CW stain. The presence and absence of hyphae were noted by each of the techniques. Second part was inoculated onto Sabouraud Dextrose agar (SDA) with and without antibiotics, along with Dermatophyte test medium (DTM). Culture plates were incubated at 30o C for four weeks. True-positive, true-negative, and diagnostic accuracy of the microscopy methods were calculated against fungal culture.
Out of 121 patients, the majority were females constituting 65.3% (n = 79) while males were 34.7% (n = 42). The average age of participants was 32.98 ± 16 years. Diagnostic accuracy was 82.6% for CSB, 87.6% for CW stain, and 76.8% for 10% KOH mount.
CW stain proved to be superior to 10% KOH mount and CSB stain for rapid dermatomycosis diagnosis, with enhanced sensitivity and diagnostic accuracy. Both CW and CSB stains are recommended for laboratory use to improve reporting accuracy as compared to the conventional 10% KOH mount technique.
Calcofluor white, Chicago sky blue, Dermatomycosis, Fungal culture, Fungal hyphae.
以真菌培养作为金标准，评估芝加哥天蓝（CSB）染色、荧光增白剂（CW）染色和氢氧化钾（KOH）涂片法对皮肤真菌病的快速诊断价值。
横断面分析研究。研究地点和时间：本研究于2023年7月至2024年2月在巴基斯坦拉瓦尔品第武装部队病理研究所/国立医学科学大学微生物学系进行。
从疑似皮肤真菌病患者处采集临床标本，包括皮肤刮屑、毛发和指甲。每个样本分为两部分。第一部分采用三种显微镜技术，即10% KOH涂片、CSB染色和CW染色。每种技术记录有无菌丝。第二部分接种于含和不含抗生素的沙氏葡萄糖琼脂（SDA）以及皮肤癣菌试验培养基（DTM）上。培养平板在30℃孵育四周。根据真菌培养结果计算显微镜检查方法的真阳性、真阴性和诊断准确性。
121例患者中，大多数为女性，占65.3%（n = 79），男性占34.7%（n = 42）。参与者的平均年龄为32.98±16岁。CSB染色的诊断准确性为82.6%，CW染色为87.6%，10% KOH涂片为76.8%。
CW染色在皮肤真菌病快速诊断方面优于10% KOH涂片和CSB染色，具有更高的敏感性和诊断准确性。与传统的10% KOH涂片技术相比，建议实验室同时使用CW和CSB染色以提高报告准确性。
荧光增白剂；芝加哥天蓝；皮肤真菌病；真菌培养；真菌菌丝
