Lucci Tyler J, Neufarth Abigail, Gaillard Jean-François, Lucks Julius B
Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Center for Synthetic Biology, Northwestern University, 633 Clark Street, Evanston, Illinois 60208, United States.
ACS Omega. 2024 Dec 19;10(1):1188-1197. doi: 10.1021/acsomega.4c08751. eCollection 2025 Jan 14.
There is growing concern around the negative health impacts associated with contamination of drinking water by harmful chemicals. Technology that enables fast, cheap, and easy detection of ions and small molecules in drinking water is thus important for reducing the incidence of these negative health impacts. Here, we describe a sensor for detecting Cu in water that provides colorimetric results in 15 min or less and functions in a just-add-water format. The sensor contains cheap reagents including salts, buffer, oxidant, chromogen, surfactant, and optionally a chelating agent. The sensor is assembled and lyophilized for shelf-stability and field-deployment. Rehydrating the sensor with water containing Cu results in chromogen oxidation and blue color formation to visually indicate the presence of Cu. The sensor demonstrates high selectivity toward Cu against other metal cations, functionality in field samples, shelf-stability, and can be tuned to activate at different Cu threshold concentrations. This sensor thus has the potential to meet a variety of needs, such as point-of-need testing for Cu to ensure water supplies meet health guidelines, such as the United States Environmental Protection Agency's Lead and Copper Rule.
有害化学物质对饮用水的污染所带来的负面健康影响日益受到关注。因此,能够快速、廉价且简便地检测饮用水中离子和小分子的技术对于减少这些负面健康影响的发生率至关重要。在此,我们描述了一种用于检测水中铜的传感器,它能在15分钟或更短时间内给出比色结果,并且以即加水的形式发挥作用。该传感器包含廉价试剂,如盐、缓冲剂、氧化剂、显色剂、表面活性剂,以及可选的螯合剂。传感器经过组装和冻干处理以实现货架稳定性和现场部署。用含铜的水对传感器进行复水会导致显色剂氧化并形成蓝色,从而直观地表明铜的存在。该传感器对铜相对于其他金属阳离子具有高选择性,在现场样品中具有功能性,具有货架稳定性,并且可以调整为在不同的铜阈值浓度下激活。因此,这种传感器有潜力满足各种需求,例如对铜进行现场即时检测,以确保供水符合健康准则,如美国环境保护局的《铅与铜规则》。