Song Ziyang, Xu Jiepei, Tian Jingyuan, Deng Jinji, Deng Xiaojing, Peng Mengdie, Luo Wenhui, Wei Minyan, Li Yongmei, Zheng Guodong
The Fifth Affiliated Hospital, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, China.
Scientific Research Center, Guangzhou Medical University, Guangzhou 511436, China.
ACS Omega. 2024 Dec 27;10(1):1688-1704. doi: 10.1021/acsomega.4c09701. eCollection 2025 Jan 14.
The nonvolatile and volatile compounds in the peels of 13 cultivars (4 mandarins, 5 tangerines, and 4 hybrids) and 5 (sweet oranges) cultivars were analyzed. Initially, 66 volatile compounds were detected using gas chromatography-mass spectrometry (GC-MS). Tangerines were distinguished from other citrus cultivars (mandarins, sweet oranges, hybrids) by having higher volatile oil extraction rates and higher relative contents of o-Cymene, α-Terpinene, d-α-Pinene, Terpinolene, γ-Terpinene, l-β-Pinene, and 3-Thujene. Additionally, 115 nonvolatile compounds were tentatively identified using ultraperformance liquid chromatography-Q-Exactive Orbitrap tandem mass spectrometry (UPLC-Q-Exactive Orbitrap-MS). contained fewer compounds than did . Pterostilbene was detected in all tangerines but not in mandarins and hybrids, suggesting its potential as a marker compound for differentiating tangerines from other . Lastly, a high-performance liquid chromatography-photodiode array (HPLC-PDA) was used to quantify 9 major nonvolatile components. Heat map and principal component analysis showed that the contents of tangerines differed from other cultivars (sweet oranges, mandarins, and hybrids). It may be caused by the higher content of synephrine, nobiletin, tangeretin, and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone in tangerines. The study may obtain information for the application of different types of (tangerines, mandarins, or hybrids) and peels, thereby promoting their recycling.
对13个品种（4个柑橘、5个橘子和4个杂交品种）以及5个甜橙品种的果皮中的非挥发性和挥发性化合物进行了分析。最初，使用气相色谱 - 质谱联用仪（GC - MS）检测到66种挥发性化合物。橘子与其他柑橘品种（柑橘、甜橙、杂交品种）的区别在于其挥发油提取率较高，以及邻异丙基甲苯、α - 萜品烯、d - α - 蒎烯、萜品油烯、γ - 萜品烯、l - β - 蒎烯和3 - 侧柏烯的相对含量较高。此外，使用超高效液相色谱 - Q - Exactive轨道阱串联质谱仪（UPLC - Q - Exactive Orbitrap - MS）初步鉴定出115种非挥发性化合物。……所含化合物比……少。在所有橘子中均检测到紫檀芪，但在柑橘和杂交品种中未检测到，这表明它有可能作为区分橘子与其他……的标记化合物。最后，使用高效液相色谱 - 光电二极管阵列（HPLC - PDA）对9种主要非挥发性成分进行定量。热图和主成分分析表明，橘子的含量与其他品种（甜橙、柑橘和杂交品种）不同。这可能是由于橘子中辛弗林、川陈皮素、橘皮素和5 - 羟基 - 6,7,8,3',4' - 五甲氧基黄酮的含量较高所致。该研究可能为不同类型的……（橘子、柑橘或杂交品种）和果皮的应用获取信息，从而促进它们的回收利用。
