Li Jingxuan, Zou Shiqi, Yang Wanling, Peng Mengdie, Chen Baizhong, Deng Jinji, Wei Minyan, Zheng Guodong
Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, The NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital Guangzhou Medical University Guangzhou China.
Guangdong Xinbaotang Biological Technology Co., Ltd Jiangmen China.
Food Sci Nutr. 2023 Jan 4;11(3):1415-1425. doi: 10.1002/fsn3.3181. eCollection 2023 Mar.
Gas chromatograph-mass spectrometer (GC-MS), ultra-high-performance liquid chromatograph-Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS), and high-performance liquid chromatography-photodiode array detection (HPLC-PDA) were used to qualitatively and quantitatively analyze the chemical component of Citri Reticulatae Pericarpium Viride "Geqingpi" (GQP). First of all, the volatile components of GQP are identified by GC-MS. Totally 56 volatile components were determined, and γ-Terpinene (33.39%) and D-Limonene (22.95%) were the main terpenes. Secondly, UHPLC-Q-Exactive Orbitrap-MS was used for identifying nonvolatile compositions and 42 compositions were identified totally, including 23 flavonoids, nine organic acids, three coumarins, two alkaloids compounds, and five other compounds, among which nine of the determined constituents were detected for the first time in GQP. Thirdly, the content of seven main constituents in GQP was quantitatively analyzed via HPLC-PDA, which were synephrine, hesperidin, limonin, nobiletin, HMF, tangeretin, and 5-HPMF. Further investigation for quantitative analysis of seven bioactive compounds suggested that the concentration of hesperidin in GQP approximately was 16.0% (160.78 ± 0.95 mg·g), which was far higher than the standard for identification and quality control of CRPV in Chinese Pharmacopoeia (2020 edition) that "the content of hesperidin shall not be less than 5.0%." The phytochemicals of GQP were elucidated in this study, which might be supporting information for identification between GQP and Citri Reticulatae Pericarpium Viride "Sihuaqingpi" (SHQP) and provided a scientific basis for the further active ingredient for pharmacological research and development prospects of GQP.
采用气相色谱 - 质谱联用仪（GC - MS）、超高效液相色谱 - Q - 高分辨质谱联用仪（UHPLC - Q - Exactive Orbitrap - MS）和高效液相色谱 - 光电二极管阵列检测法（HPLC - PDA）对绿皮“个青皮”（GQP）的化学成分进行定性和定量分析。首先，通过GC - MS鉴定GQP的挥发性成分。共测定了56种挥发性成分，其中γ - 萜品烯（33.39%）和D - 柠檬烯（22.95%）是主要的萜类成分。其次，采用UHPLC - Q - Exactive Orbitrap - MS鉴定非挥发性成分，共鉴定出42种成分，包括23种黄酮类、9种有机酸、3种香豆素、2种生物碱类化合物以及5种其他化合物，其中有9种测定成分在GQP中首次被检测到。第三，通过HPLC - PDA对GQP中7种主要成分进行定量分析，这7种成分分别是辛弗林、橙皮苷、柠檬苦素、川陈皮素、5 - 羟甲基糠醛、橘红素和5 - 羟基 - 3,6,7,8,3',4' - 六甲氧基黄酮。对7种生物活性化合物的进一步定量分析表明，GQP中橙皮苷的含量约为16.0%（160.78 ± 0.95 mg·g），远高于《中国药典》（2020年版）中绿皮（CRPV）鉴别和质量控制标准“橙皮苷含量不得少于5.0%”。本研究阐明了GQP的植物化学物质，这可能为GQP与绿皮“四化青皮”（SHQP）的鉴别提供辅助信息，并为GQP药理研发前景的进一步活性成分研究提供科学依据。
