Identification of volatile and nonvolatile compounds in Citri Reticulatae Pericarpium Viride using GC-MS, UPLC-Q-Exactive Orbitrap-MS, and HPLC-PDA.

Gas chromatograph-mass spectrometer (GC-MS), ultra-high-performance liquid chromatograph-Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS), and high-performance liquid chromatography-photodiode array detection (HPLC-PDA) were used to qualitatively and quantitatively analyze the chemical component of Citri Reticulatae Pericarpium Viride "Geqingpi" (GQP). First of all, the volatile components of GQP are identified by GC-MS. Totally 56 volatile components were determined, and γ-Terpinene (33.39%) and D-Limonene (22.95%) were the main terpenes. Secondly, UHPLC-Q-Exactive Orbitrap-MS was used for identifying nonvolatile compositions and 42 compositions were identified totally, including 23 flavonoids, nine organic acids, three coumarins, two alkaloids compounds, and five other compounds, among which nine of the determined constituents were detected for the first time in GQP. Thirdly, the content of seven main constituents in GQP was quantitatively analyzed via HPLC-PDA, which were synephrine, hesperidin, limonin, nobiletin, HMF, tangeretin, and 5-HPMF. Further investigation for quantitative analysis of seven bioactive compounds suggested that the concentration of hesperidin in GQP approximately was 16.0% (160.78 ± 0.95 mg·g), which was far higher than the standard for identification and quality control of CRPV in Chinese Pharmacopoeia (2020 edition) that "the content of hesperidin shall not be less than 5.0%." The phytochemicals of GQP were elucidated in this study, which might be supporting information for identification between GQP and Citri Reticulatae Pericarpium Viride "Sihuaqingpi" (SHQP) and provided a scientific basis for the further active ingredient for pharmacological research and development prospects of GQP.