环黄芪醇促进小鼠背柱轴突再生。
Cycloastragenol promotes dorsal column axon regeneration in mice.
作者信息
Zihan Tao, Wenwen Teng, Yanxia Ma
机构信息
Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, School of Medicine, Hangzhou City University, Hangzhou, China.
Orthopaedic Institute, Suzhou Medical College, Soochow University, Suzhou, Jiangsu, China.
出版信息
Front Cell Neurosci. 2025 Jan 3;18:1424137. doi: 10.3389/fncel.2024.1424137. eCollection 2024.
INTRODUCTION
Cycloastragenol (CAG) has a wide range of pharmacological effects, including anti-inflammatory, antiaging, antioxidative, and antitumorigenic properties. In addition, our previous study showed that CAG administration can promote axonal regeneration in peripheral neurons. However, whether CAG can activate axon regeneration central nervous system (CNS) remains unknown.
METHODS
Here, we established a novel mouse model for visualizing spinal cord dorsal column axon regeneration involving the injection of AAV2/9-Cre into the lumbar 4/5 dorsal root ganglion (DRG) of Rosa-tdTomato reporter mice. We then treated mice by intraperitoneal administration of CAG.
RESULTS
Our results showed that intraperitoneal CAG injections significantly promoted the growth of -cultured DRG axons as well as the growth of dorsal column axons over the injury site in spinal cord injury (SCI) mice. Our results further indicate that CAG administration can promote the recovery of sensory and urinary function in SCI mice.
CONCLUSION
Together, our findings highlight the therapeutic potential of CAG in spinal cord injury repair.
引言
环黄芪醇（CAG）具有广泛的药理作用，包括抗炎、抗衰老、抗氧化和抗肿瘤特性。此外，我们之前的研究表明，给予CAG可促进外周神经元的轴突再生。然而，CAG是否能激活中枢神经系统（CNS）的轴突再生仍不清楚。
方法
在此，我们建立了一种新型小鼠模型，用于可视化脊髓背柱轴突再生，该模型涉及将AAV2/9-Cre注射到Rosa-tdTomato报告基因小鼠的腰4/5背根神经节（DRG）中。然后我们通过腹腔注射CAG对小鼠进行治疗。
结果
我们的结果表明，腹腔注射CAG可显著促进培养的DRG轴突生长以及脊髓损伤（SCI）小鼠损伤部位上方背柱轴突的生长。我们的结果进一步表明，给予CAG可促进SCI小鼠感觉和泌尿功能的恢复。
结论
总之，我们的研究结果突出了CAG在脊髓损伤修复中的治疗潜力。
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