环黄芪醇对牛胚胎发育、着床潜能和端粒酶活性的影响。

The effects of cycloastragenol on bovine embryo development, implantation potential and telomerase activity.

机构信息

Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province 52828, Republic of Korea.

Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Gyeongnam Province 52828, Republic of Korea; and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam Province, Republic of Korea.

出版信息

Reprod Fertil Dev. 2023 Jun;35(10):527-538. doi: 10.1071/RD22280.

DOI:10.1071/RD22280

PMID:37263290

Abstract

CONTEXT

Telomerase reverse transcriptase is a key factor responsible for structural and cellular alterations in aged oocytes and changes in the structure of the zona pellucida and mitochondria. Telomerase expression is reduced in aged cumulus oocyte complexes, and its activation or enhanced expression would be beneficial for in vitro oocyte maturation and in vitro embryo development.

AIMS

This study aimed to investigate telomerase activation by cycloastragenol and its effect on bovine oocyte in vitro maturation, fertilisation, and early embryo development.

METHODS

We used qPCR, Western blot, immunofluorescence, reactive oxygen species (ROS) assay,TUNEL assay, JC-1 assay, and invasion assay to analyse the affect of cycloastragenol (CAG) on bovine oocyte maturation, embryo development, embryo quality and implantation potential.

KEY RESULTS

Cycloastragenol treatment of oocytes in in vitro maturation (IVM) media significantly (P <0.05) improved oocyte IVM (90.87%), embryo cleavage (90.78%), blastocyst hatching (27.04%), and embryo implantation potential. Telomerase also interacts with mitochondria, and JC-1 staining results showed significantly (P <0.05) higher mitochondrial membrane potential (ΔΨ m) in the CAG-treated group. Furthermore, the inner cell mass (OCT4 and SOX2) and trophoblasts (CDX2) of the control and CAG groups were examined. Moreover, CAG treatment to primary cultured bovine cumulus cells substantially enhanced telomerase activity.

CONCLUSIONS

Telomerase activation via cycloastragenol is beneficial for bovine oocyte IVM and for the production of high-quality bovine embryos.

IMPLICATIONS

Cycloastragenol is a natural telomerase activator, and could be useful as a permanent component of oocyte maturation media.

摘要

背景

端粒酶逆转录酶是导致老化卵母细胞结构和细胞改变以及透明带和线粒体结构改变的关键因素。端粒酶在老化的卵丘卵母细胞复合物中的表达减少，其激活或增强表达将有利于体外卵母细胞成熟和体外胚胎发育。

目的

本研究旨在探讨环黄芪醇对牛卵母细胞体外成熟、受精和早期胚胎发育的端粒酶激活作用。

方法

我们使用 qPCR、Western blot、免疫荧光、活性氧（ROS）测定、TUNEL 测定、JC-1 测定和侵袭测定来分析环黄芪醇（CAG）对牛卵母细胞成熟、胚胎发育、胚胎质量和植入潜能的影响。

主要结果

在体外成熟（IVM）培养基中添加环黄芪醇处理卵母细胞，可显著（P <0.05）提高卵母细胞 IVM（90.87%）、胚胎卵裂（90.78%）、囊胚孵化（27.04%）和胚胎着床潜能。端粒酶还与线粒体相互作用，JC-1 染色结果显示 CAG 处理组线粒体膜电位（ΔΨ m）显著升高（P <0.05）。此外，还检测了对照组和 CAG 组的内细胞团（OCT4 和 SOX2）和滋养层（CDX2）。此外，CAG 处理原代培养的牛卵丘细胞可显著增强端粒酶活性。