Rizvi Syed Ahmed, Chowdhury Kaniz F, Borsetto Chiara, Travis Emma R, Saif Mohammad, Ahmed Vikar, Carter Laura J, Ahmad Iqbal, McNally Alan, Haq Qazi Mohd Rizwanul
Department of Biosciences, Jamia Millia Islamia, New Delhi, India.
School of Geography, Faculty of Environment, University of Leeds, Leeds, UK.
MethodsX. 2024 Dec 22;14:103122. doi: 10.1016/j.mex.2024.103122. eCollection 2025 Jun.
Microbial pathogens have developed resistance mechanisms to almost every antibiotic available. There is a need to synthesize or screen new natural compounds to combat the development of drug-resistant pathogens. One of the commonly used methods to evaluate the antimicrobial activity of two or more antibiotics involves a checkerboard assay, which is cumbersome, time-consuming, and expensive. We have developed a quick, reliable, and cost-effective method to evaluate the antimicrobial effect of two or more antibiotics at fixed doses with different concentrations of a novel natural ingredient or test compound. The technique involves the following steps:•Preparation of a bacterial culture of the test strain at 0.5 McFarland standard (0.1 OD at 600 nm), and preparation of stock solutions for the chemical of interest and standard drugs.•The required amount of all three components can be dispensed into respective wells of a microplate using multichannel pipette.•Optical density (OD) values obtained would be directly related to the individual as well as combined effect of compounds on the given bacterial strain.
微生物病原体已对几乎所有可用的抗生素产生了耐药机制。因此需要合成或筛选新的天然化合物来对抗耐药病原体的产生。评估两种或更多种抗生素抗菌活性的常用方法之一是棋盘试验，该方法繁琐、耗时且昂贵。我们开发了一种快速、可靠且经济高效的方法，用于在固定剂量下，用不同浓度的新型天然成分或测试化合物评估两种或更多种抗生素的抗菌效果。该技术包括以下步骤：
• 将测试菌株的细菌培养物制备成0.5麦氏标准（600nm处吸光度为0.1），并制备感兴趣化学品和标准药物的储备溶液。
• 可以使用多通道移液器将所需量的所有三种成分分配到微孔板的各个孔中。
• 获得的光密度（OD）值将直接与化合物对给定细菌菌株的单独以及联合作用相关。
