Pipponzi Sara, Primisser Stefanie, Antonielli Livio, Stefani Emilio, Compant Stephane, Sessitsch Angela, Kostic Tanja
Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy.
Center for Health & Bioresources, Bioresources Unit, AIT Austrian Institute of Technology, Tulln, Austria.
J Sci Food Agric. 2025 Apr;105(6):3343-3354. doi: 10.1002/jsfa.14095. Epub 2025 Jan 20.
Vitamin B (cobalamin) can be produced de novo only by certain bacteria and archaea. It plays a crucial role in the health of animals and humans, which obtain it only through diet, mainly from animal products. This study aimed to identify endophytic bacterial strains capable of synthesizing vitamin B and enriching edible plants with it as a potential solution for vitamin B deficiency in vegetarians, vegans, and people with poor diets.
An in silico genome analysis was performed on 66 bacterial genomes, including the reference strain Pseudomonas denitrificans ATCC 13867, a known vitamin B producer. The genomes were analyzed using the Rapid Annotations using Subsystems Technology (RAST) server and the MetaCyc database to verify the presence and completeness of the vitamin B metabolic pathway. The ability of the strains to produce vitamin B was confirmed with a high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis of pure culture extracts. Eleven strains produced detectable amounts of vitamin B under tested conditions. The best performing candidates were further tested for their efficacy in producing vitamin B in lettuce grown under sterile conditions on Murashige and Skoog (MS) medium with or without CoCl supplementation. Methylobacterium sp. strain P1-11 produced detectable amounts of vitamin B in planta: 1.654 and 2.559 μg per g of dry weight without and with CoCl supplementation, respectively.
This is the first time a bacterial endophyte was used to produce vitamin B in planta, suggesting that bacterial endophytes could be utilized to enhance the nutraceutical values of plant-based foods. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
维生素B（钴胺素）只能由某些细菌和古生菌从头合成。它对动物和人类的健康起着至关重要的作用，而动物和人类只能通过饮食获取维生素B，主要来自动物产品。本研究旨在鉴定能够合成维生素B并使可食用植物富含该维生素的内生细菌菌株，作为素食者、纯素食者和饮食不良者维生素B缺乏的潜在解决方案。
对66个细菌基因组进行了计算机基因组分析，包括参考菌株反硝化假单胞菌ATCC 13867，一种已知的维生素B生产者。使用基于子系统技术的快速注释（RAST）服务器和MetaCyc数据库对基因组进行分析，以验证维生素B代谢途径的存在和完整性。通过对纯培养提取物的高效液相色谱-二极管阵列检测（HPLC-DAD）分析，证实了菌株产生维生素B的能力。11株菌株在测试条件下产生了可检测量的维生素B。对表现最佳的候选菌株进一步测试其在添加或不添加CoCl的Murashige和Skoog（MS）培养基上无菌培养的生菜中产生维生素B的功效。甲基杆菌属菌株P1-11在植物中产生了可检测量的维生素B：不添加CoCl时为每克干重1.654μg，添加CoCl时为每克干重2.559μg。
这是首次利用细菌内生菌在植物中产生维生素B，表明细菌内生菌可用于提高植物性食品的营养保健价值。© 2025作者。由John Wiley & Sons Ltd代表化学工业协会出版的《食品与农业科学杂志》。
