Zhang Jizhao, Zhao Qiang
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
School of Environment, Hangzhou Institute for Advanced Study, UCAS, Hangzhou, 310024, China.
Anal Bioanal Chem. 2025 Mar;417(8):1441-1448. doi: 10.1007/s00216-025-05733-2. Epub 2025 Jan 20.
Chloramphenicol (CAP) is widely used in treating bacteria infection in animals and humans. However, the accumulation of CAP in food and environment caused serious health risk to human. Consequently, sensitive and selective detection of CAP is of great importance in environmental monitoring and food safety. Among various analytical methods, aptamer-based biosensors exhibit great potentials for CAP detection. Here, we developed an aptamer-based biosensor for rapid fluorescence detection of CAP based on pyrene excimer switch by using a newly selected short DNA aptamer with high affinity. The aptamer was labeled with pyrene molecules at both ends. The binding of CAP to the aptamer probe caused two pyrene molecules close to each other and the formation of a pyrene excimer, which induced the increase of the fluorescence signal from the pyrene excimer. CAP detection was achieved by measuring the fluorescence signal changes of the aptamer probes with dual pyrene labels. Under optimized conditions, the developed aptamer biosensor showed a detection limit of 24.4 nmol/L for CAP. The aptamer-based fluorescence sensor could quantify CAP in diluted tap water and lake water, exhibiting potentials for the application in real sample sensing of CAP.
氯霉素（CAP）被广泛用于治疗人和动物的细菌感染。然而，CAP在食品和环境中的积累对人类健康造成了严重风险。因此，灵敏且选择性地检测CAP在环境监测和食品安全方面具有重要意义。在各种分析方法中，基于适配体的生物传感器在CAP检测方面展现出巨大潜力。在此，我们利用新筛选出的具有高亲和力的短DNA适配体，基于芘激基缔合物开关开发了一种用于快速荧光检测CAP的基于适配体的生物传感器。该适配体两端均标记有芘分子。CAP与适配体探针的结合使两个芘分子彼此靠近并形成芘激基缔合物，这导致芘激基缔合物的荧光信号增强。通过测量带有双芘标记的适配体探针的荧光信号变化来实现CAP检测。在优化条件下，所开发的基于适配体的生物传感器对CAP的检测限为24.4 nmol/L。基于适配体的荧光传感器能够对稀释的自来水和湖水中的CAP进行定量，在CAP实际样品传感中展现出应用潜力。
