State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China; University of Chinese Academy of Sciences, Beijing 10049, PR China.
Biosens Bioelectron. 2016 Jul 15;81:454-459. doi: 10.1016/j.bios.2016.03.034. Epub 2016 Mar 16.
A novel fluorescence aptasensor was successfully developed to respond to chloramphenicol (CAP) in food based on magnetic aptamer-liposome vesicle probe. In order to fabricate it, aptamer labeled on functionalized magnetic beads (MB) was firstly employed as capture adsorbent (MB-Apt), then SSB (single-stranded DNA binding protein) and DIL (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanineperchlorate) coimmobilized liposomes (SSB/DIL-Lip) was employed as vesicle signal tracer. The composite vesicle probe is formed between SSB/DIL-Lip and MB-Apt based on SSB's specific recognition towards aptamer on vesicle signal tracer. Upon the vesicle probe solution reacted with CAP, the aptamer on the magnetic beads preferentially bounded with CAP, and then released SSB/DIL-Lip vesicle signal tracer in the supernatant after magnetic separation. The released tracer can emit fluorescence which was correspondence with the concentration of the analyte. At the optimum conditions, the aptasensor exhibited a good linear response for CAP detection in the range of 0.003-10nM with a detection limit of 1pM. Importantly, the methodology was further validated for analyzing CAP in fish samples with consistent results obtained by ELISA kit, thus providing a promising approach for quantitative monitoring of CAP and significant anti-interference ability in food safety.
一种基于磁性适体-脂质体囊泡探针的新型荧光适体传感器成功地被开发出来，用于响应食品中的氯霉素 (CAP)。为了制备它，首先将标记在功能化磁珠 (MB) 上的适体用作捕获吸附剂 (MB-Apt)，然后将 SSB（单链 DNA 结合蛋白）和 DIL（1,1'-十八烷基-3,3,3',3'-四甲基吲哚碳菁高氯酸盐）共固定化脂质体 (SSB/DIL-Lip) 用作囊泡信号示踪剂。在 SSB 对囊泡信号示踪剂上的适体的特异性识别作用下，SSB/DIL-Lip 和 MB-Apt 之间形成了复合囊泡探针。当囊泡探针溶液与 CAP 反应时，磁珠上的适体优先与 CAP 结合，然后在磁分离后将 SSB/DIL-Lip 囊泡信号示踪剂释放到上清液中。释放的示踪剂可以发出荧光，荧光强度与分析物的浓度相对应。在最佳条件下，该适体传感器对 CAP 的检测范围为 0.003-10nM，检测限为 1pM，具有良好的线性响应。重要的是，该方法进一步用于分析鱼类样品中的 CAP，与 ELISA 试剂盒得到的结果一致，从而为 CAP 的定量监测提供了一种有前途的方法，并具有显著的食品安全抗干扰能力。
