Song Yu-Ting, Sitthikitpanya Napapat, Usmanbaha Nikannapas, Reungsang Alissara, Chu Chen-Yeon
Master's Program of Green Energy Science and Technology, Feng Chia University, Taichung City, 407102, Taiwan.
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand.
Biodegradation. 2025 Jan 20;36(1):14. doi: 10.1007/s10532-025-10110-y.
Bioplastics, particularly polyhydroxyalkanoates (PHAs), are emerging as promising alternatives to traditional materials due to their biodegradability. This study focuses on the production of PHAs as bioplastics using effluent from hydrogen production in a two-stage Biohythane Pilot Plant, which provides a low-cost substrate. The aim is to optimize production conditions, with Cupriavidus necator TISTR 1335 being used as the PHA producer. Utilizing Response Surface Methodology-Central Composite Design, we explored optimal conditions, revealing peak PHA production at a substrate concentration of 33.51 g COD/L and a pH of 6.87. The predicted optimal PHA concentration was at 3.05 g/L within the established model, closely matching the experimentally validated value of 3.02 g/L, with the overall usage rate of reducing sugars approximately 50-60%. This study underscores the importance of optimizing PHA production conditions and paving the way toward large-scale PHA production.
生物塑料，特别是聚羟基脂肪酸酯（PHA），由于其可生物降解性，正成为传统材料的有前途的替代品。本研究重点关注在两阶段生物制氢中试装置中利用制氢废水生产作为生物塑料的PHA，该废水提供了低成本的底物。目的是优化生产条件，使用食酸铜绿假单胞菌TISTR 1335作为PHA生产者。利用响应面法-中心复合设计，我们探索了最佳条件，发现在底物浓度为33.51 g COD/L和pH值为6.87时PHA产量达到峰值。在建立的模型中，预测的最佳PHA浓度为3.05 g/L，与实验验证值3.02 g/L非常接近，还原糖的总体利用率约为50-60%。本研究强调了优化PHA生产条件的重要性，并为大规模生产PHA铺平了道路。
