Kumar Mukesh, Sachan Rohan Samir Kumar, Karnwal Arun, Sur Dharmesh, Thakur Rahul, Kumar Abhinav, Manickkam Sathiyamoorthy, Ayanie Abinet Gosaye, Almujibah Hamad
Department of Medical Laboratory Sciences, GNA University, Phagwara, 144401, Punjab, India.
Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, 144411, Punjab, India.
Sci Rep. 2025 May 25;15(1):18205. doi: 10.1038/s41598-025-01305-7.
The valorization of wood waste as a sustainable bacterial feedstock for the production of Polyhydroxybutyrate (PHB) is explored in this study, aiming to provide an environmentally friendly alternative to conventional plastics. Wood waste, treated with 4% sulfuric acid, served as the carbon source for isolating bacteria from Jalandhar waste streams, with the strain Klebsiella sp. MK3 identified as the most effective in PHB production after 16s rRNA sequencing. Analytical methods including the Molisch test, DNS, and sugar utilization tests confirmed sugar presence and consumption by the bacterial isolate. Media optimization using Design Expert 12.0 utilized a quadratic model, achieving a robust fit with an R² value of 98.6%. Optimization via Plackett-Burman design and response surface methodology enhanced PHB yield to 4.37 mg/mL, a significant increase over previous benchmarks. This yield was achieved under optimal conditions of 1.7% carbon concentration, 0.105% nitrogen concentration, and a constant temperature of 37 °C. Qualitative analysis of PHB by UV-Vis spectroscopy, FTIR, and NMR confirmed its purity and composition. The study highlights the potential of wood waste and wastewater as substrates for cost-effective PHB production, with significant applications in packaging, agriculture, medicine, and more, thus promoting reduced reliance on non-renewable resources and advancing sustainability goals.
本研究探索了将木材废料作为生产聚羟基丁酸酯（PHB）的可持续细菌原料的价值，旨在为传统塑料提供一种环境友好的替代品。用4%硫酸处理过的木材废料作为从贾朗达尔废水中分离细菌的碳源，经16s rRNA测序后，克雷伯氏菌属MK3菌株被确定为生产PHB最有效的菌株。包括莫利施试验、DNS和糖利用试验在内的分析方法证实了细菌分离物中糖的存在和消耗情况。使用Design Expert 12.0进行培养基优化采用了二次模型，R²值为98.6%，拟合效果良好。通过Plackett-Burman设计和响应面方法进行优化，使PHB产量提高到4.37 mg/mL，比之前的基准有显著提高。该产量是在1.7%的碳浓度、0.105%的氮浓度和37°C的恒温最佳条件下实现的。通过紫外可见光谱、傅里叶变换红外光谱和核磁共振对PHB进行定性分析，证实了其纯度和组成。该研究突出了木材废料和废水作为经济高效生产PHB的底物的潜力，在包装、农业、医药等领域有重要应用，从而减少对不可再生资源的依赖，推进可持续发展目标。
