Akram Mohammad, Kumar Deepender, Saurav Sonu, Saxena Moly, Saxena Deep Ratna, Kamaal Naimuddin, Dixit Girish Prasad
Division of Crop Protection, ICAR-Indian Institute of Pulses Research, Kanpur, 208024, India.
Department of Plant Pathology, R.A.K. College of Agriculture, Sehore, 466001, India.
Curr Microbiol. 2025 Jan 20;82(2):95. doi: 10.1007/s00284-025-04080-w.
Pigeonpea (Cajanus cajan L.) plants exhibiting symptoms of yellow mosaic disease (YMD) were collected in winter 2023 from multiple agricultural fields of Kanpur, Sehore, and Madhubani, representing three different agro-ecological zones in India. The recorded disease incidence ranged from 3 to 5%, 1 to 4%, and 12 to 20% in these zones, respectively. This study aimed to identify and characterize the causal agent, suspected to be a begomovirus, an emerging plant pathogen of pigeonpea causing YMD. Total DNA was extracted from 28 YMD-affected leaf samples and subjected to rolling circle amplification for PCR-based virus detection. Of all the tested samples, one tested positive for mungbean yellow mosaic virus (MYMV), while the remaining tested positive for mungbean yellow mosaic India virus (MYMIV). Subsequently, PCR-based amplification and sequencing of the full-length DNA-A and DNA-B components were conducted. BLASTn analysis revealed that the assembled sequences of the DNA-A and DNA-B components had the highest nucleotide identity with MYMIV (DNA-A: 97-99%, DNA-B: 95-97%) and MYMV (DNA-A: 99%, DNA-B: 98%). Phylogenetic analysis supported these findings. Additionally, the DNA-A and DNA-B components obtained from each sample were found to be cognate, with over 92% similarity in their common region. Thus, the cognate DNA components constituted the isolates of MYMIV and MYMV identified from pigeonpea. The identified isolates exhibited the typical genome organization of an Old World bipartite begomovirus, with no recombination events detected. This study reports, for the first time, the complete annotated genomes of MYMIV from Sehore and Madhubani, as well as MYMIV and MYMV from Kanpur, infecting pigeonpea.
2023年冬季，从印度坎普尔、塞霍雷和马图巴尼的多个农田收集了表现出黄花叶病（YMD）症状的木豆（Cajanus cajan L.）植株，这些农田代表了印度三个不同的农业生态区。这些区域记录的发病率分别为3%至5%、1%至4%和12%至20%。本研究旨在鉴定和表征疑似菜豆金色花叶病毒属病毒的致病因子，该病毒是一种新兴的导致木豆黄花叶病的植物病原体。从28个受黄花叶病影响的叶片样本中提取总DNA，并进行滚环扩增以进行基于PCR的病毒检测。在所有测试样本中，一个样本对绿豆黄花叶病毒（MYMV）检测呈阳性，其余样本对印度绿豆黄花叶病毒（MYMIV）检测呈阳性。随后，对全长DNA-A和DNA-B组件进行基于PCR的扩增和测序。BLASTn分析显示，DNA-A和DNA-B组件的组装序列与MYMIV（DNA-A：97-99%，DNA-B：95-97%）和MYMV（DNA-A：99%，DNA-B：98%）具有最高的核苷酸同一性。系统发育分析支持了这些发现。此外，从每个样本中获得的DNA-A和DNA-B组件被发现是同源的，其共同区域的相似度超过92%。因此，同源DNA组件构成了从木豆中鉴定出的MYMIV和MYMV分离株。鉴定出的分离株表现出旧大陆二分体菜豆金色花叶病毒属病毒的典型基因组结构，未检测到重组事件。本研究首次报告了来自塞霍雷和马图巴尼的MYMIV以及来自坎普尔的MYMIV和MYMV感染木豆的完整注释基因组。
