傅里叶变换红外振动光谱法在识别接受Roux-en-y胃旁路手术个体脂质谱早期生化变化中的应用

Application of fourier transform infrared vibrational spectroscopy in identifying early biochemical changes in lipid profiles of individuals undergoing Roux-en-y gastric bypass.

作者信息

Motta de Bortoli Amanda, Cassago Nascimento Márcia Helena, Guerrero Daboin Blanca Elena, Bobbio de Brito Beatriz, Recla Pessotti Luiza, Filgueiras Paulo Roberto, Bolsoni Lopes Andressa, Garrone Barauna Valerio, Kenji Haraguchi Fabiano

机构信息

Postgraduate Program in Nutrition and Health, Health Sciences Center, Federal University of Espírito Santo, Av. Mal. Campos, 1468 - Maruípe, Vitória, 29047-100, ES, Brazil.

Postgraduate Program in Chemistry, Center of Exact Sciences, Federal University of Espírito Santo, Av. Fernando Ferrari, 514 - Goiabeiras, Vitória, 29075-910, ES, Brazil.

出版信息

BMC Surg. 2025 Jan 20;25(1):33. doi: 10.1186/s12893-024-02707-6.

DOI:10.1186/s12893-024-02707-6

PMID:39833830

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11748525/

Abstract

BACKGROUND

Fourier transform infrared spectroscopy (FTIR) is an analytical technique increasingly applied in biological analysis. This study investigates the application of FTIR to identify early biochemical changes, particularly in lipid profiles, in individuals undergoing Roux-en-Y gastric bypass (RYGB).

METHODS

An observational study involving patients from a university hospital's Bariatric and Metabolic Surgery Program, with evaluations performed before (T0) and two months after (T1) RYGB. Biochemical parameters, anthropometric data, and body composition were assessed. FTIR spectra were pre-processed and analyzed using Principal Component Analysis and Partial Least Squares Discriminant Analysis. The normality of the data was evaluated using the Kolmogorov-Smirnov test, followed by paired T-tests or Wilcoxon tests as appropriate. Spearman correlation analysis of spectral information with biochemical parameters was also performed. A significance level of p < 0.05 was set for all tests. The university hospital's Research Ethics Committee approved the study (protocol CAAE 59075722.7.0000.5071).

RESULTS

The study evaluated 29 individuals (86.2% female) with a mean age of 41.2 ± 7.8 years. Significant differences were observed in anthropometric parameters and body composition (p < 0.001). Additionally, early improvements in the lipid profile were noted, with significant decreases in triglycerides, total cholesterol, and LDL cholesterol (p < 0.05) just two months post-surgery. FTIR identified correlations between biochemical parameters and specific spectral regions at T0 and T1. Notably, serum triglycerides showed a significant correlation with the lipid-specific spectral region (1796-1685 cm) at both time points (p < 0.05).

CONCLUSION

FTIR can effectively monitor biochemical changes in RYGB surgery patients. The spectral range associated with lipid functional groups (1796 -1675 cm⁻¹) showed a significant relationship with serum triglyceride levels before and after RYGB. Additionally, various biochemical parameters exhibited strong correlations with other spectral regions, implying that the serum spectral profile can indicate biochemical variations at different post-surgery stages.

TRIAL REGISTRATION

Brazilian Registry of Clinical Trials (Rebec), September 5, 2022, protocol RBR-26chs2g.

摘要

背景

傅里叶变换红外光谱（FTIR）是一种在生物分析中应用日益广泛的分析技术。本研究调查了FTIR在识别接受Roux-en-Y胃旁路术（RYGB）的个体早期生化变化，特别是脂质谱变化方面的应用。

方法

一项观察性研究，涉及某大学医院肥胖与代谢手术项目的患者，在RYGB术前（T0）和术后两个月（T1）进行评估。评估生化参数、人体测量数据和身体成分。对FTIR光谱进行预处理，并使用主成分分析和偏最小二乘判别分析进行分析。使用Kolmogorov-Smirnov检验评估数据的正态性，然后根据情况进行配对t检验或Wilcoxon检验。还对光谱信息与生化参数进行了Spearman相关性分析。所有检验的显著性水平设定为p < 0.05。该大学医院的研究伦理委员会批准了本研究（协议编号CAAE 59075722.7.0000.5071）。

结果

该研究评估了29名个体（86.2%为女性），平均年龄为41.2 ± 7.8岁。在人体测量参数和身体成分方面观察到显著差异（p < 0.001）。此外，注意到脂质谱的早期改善，术后仅两个月，甘油三酯、总胆固醇和低密度脂蛋白胆固醇显著降低（p < 0.05）。FTIR确定了T0和T1时生化参数与特定光谱区域之间的相关性。值得注意的是，血清甘油三酯在两个时间点均与脂质特异性光谱区域（1796 - 1685 cm）显示出显著相关性（p < 0.05）。