Han Xiu Feng, Che Ming Yue, Su Xue, Tian Jing Yun, Liu Lan Hao, Nie Pin, Wang Su
School of Marine Science and Engineering, Qingdao Agricultural University, 700 Greatwall Road, Qingdao, Shandong Province, 266109, China.
Marine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao), Qingdao, Shandong Province, 266104, China.
Fish Shellfish Immunol. 2025 Mar;158:110135. doi: 10.1016/j.fsi.2025.110135. Epub 2025 Jan 19.
Tumor necrosis factor receptor-associated factors (TRAFs) act as signal transducers and are critical in many biological processes. However, in contrast to mammals, the function of TRAFs in teleost is still largely unknown. In this study, we identified and cloned eight TRAF genes in snakehead (Channa argus), namely CaTRAF2aa, CaTRAF2ab, CaTRAF2b, CaTRAF3, CaTRAF4a, CaTRAF5, CaTRAF6, and CaTRAF7. Bioinformatics analyses exhibited CaTRAF genes were evolutionarily conserved among teleost. Subcellular localization results demonstrated that eight CaTRAFs were all localized in cytoplasm. These CaTRAFs showed widespread but different expression profiles in various organs/tissues of snakehead and their expression could be induced by IHSV infection. Furthermore, almost all CaTRAFs can be impacted by poly (I:C) stimulation in HKLs. To sum up, this study provides a valuable foundation for further functional research on teleost TRAF genes.
肿瘤坏死因子受体相关因子（TRAFs）作为信号转导分子，在许多生物学过程中起关键作用。然而，与哺乳动物不同，TRAFs在硬骨鱼中的功能仍 largely未知。在本研究中，我们在乌鳢（Channa argus）中鉴定并克隆了八个TRAF基因，即CaTRAF2aa、CaTRAF2ab、CaTRAF2b、CaTRAF3、CaTRAF4a、CaTRAF5、CaTRAF6和CaTRAF7。生物信息学分析表明，TRAF基因在硬骨鱼中具有进化保守性。亚细胞定位结果表明，八个CaTRAFs均定位于细胞质中。这些CaTRAFs在乌鳢的各种器官/组织中表现出广泛但不同的表达谱，并且它们的表达可被IHSV感染诱导。此外，几乎所有CaTRAFs都可受到HKLs中聚（I:C）刺激的影响。总之，本研究为硬骨鱼TRAF基因的进一步功能研究提供了有价值的基础。
