Sun Chaonan, Zhu Mingxin, Wang Lingyu, Wen Haishen, Qi Xin, Li Chao, Zhang Xiaoyan, Sun Donglei, Li Yun
Key Laboratory of Mariculture (Ocean University of China), Ministry of Education (KLMME), Fisheries College, Ocean University of China, Qingdao, 266003, China.
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
Fish Shellfish Immunol. 2025 Feb;157:110076. doi: 10.1016/j.fsi.2024.110076. Epub 2024 Dec 5.
The mitogen-activated protein kinase (MAPK) signaling cascade, integral to cellular regulation, orchestrates cell growth, differentiation, stress response, and inflammatory reactions to adapt to challenging environments. The northern snakeheads (Channa argus), a valuable freshwater species known for its hypoxia tolerance, rapid growth, and high nutritional value, lacks comprehensive research on its mapk gene family. In this study, we identified 16 mapk genes in northern snakeheads, among which mapk8, mapk12 and mapk14 have duplicate copies. Phylogenetic analysis confirmed the evolutionary conservation of this gene family. Structural and motif analyses further underscored the conserved nature of these genes. Expression pattern analysis under abiotic and biotic stress conditions showed significant differences expression of mapks in the gills and suprabranchial organ (SBO) after air exposure, as well as in the brain following cold stress, highlighting the extensive role of mapks in stress regulation. It was worth noting that the significant expression differences of mapks were also observed in the spleen after N. seriolae infection, implicating that these genes may be involved in the regulation of innate immune responses. Additionally, analysis of protein-protein interaction (PPI) networks suggested that the co-activation of multiple MAPK signaling pathways may play a key role in regulating an organism's response to biotic and abiotic stresses. This study provides a detailed description of the mapk gene family in the northern snakeheads and elucidates its biological functions under various stress conditions, offering valuable insights into the regulatory mechanisms of the mapk gene family.
丝裂原活化蛋白激酶（MAPK）信号级联是细胞调节不可或缺的一部分，它协调细胞生长、分化、应激反应和炎症反应，以适应具有挑战性的环境。乌鳢（Channa argus）是一种珍贵的淡水物种，以其耐缺氧、生长迅速和营养价值高而闻名，但对其mapk基因家族缺乏全面研究。在本研究中，我们在乌鳢中鉴定出16个mapk基因，其中mapk8、mapk12和mapk14有重复拷贝。系统发育分析证实了该基因家族的进化保守性。结构和基序分析进一步强调了这些基因的保守性质。在非生物和生物应激条件下的表达模式分析表明，空气暴露后鳃和鳃上器官（SBO）中的mapks以及冷应激后脑中的mapks表达存在显著差异，突出了mapks在应激调节中的广泛作用。值得注意的是，在感染鰤诺卡氏菌后脾脏中也观察到mapks的显著表达差异，这表明这些基因可能参与先天免疫反应的调节。此外，蛋白质-蛋白质相互作用（PPI）网络分析表明，多个MAPK信号通路的共同激活可能在调节生物体对生物和非生物应激的反应中起关键作用。本研究详细描述了乌鳢中的mapk基因家族，并阐明了其在各种应激条件下的生物学功能，为mapk基因家族的调控机制提供了有价值的见解。
