Zhang Qiuli, Hong Tao, Su Tian, Li Qiaolong, Xiang Aoni, Chen Xinlong, He Guanghua, Zhao Fangming, Wu Renhong
Key Laboratory of Crop Molecular Improvement, Rice Research Institute, Academy of Agricultural Sciences, Ministry of Education, Southwest University, Chongqing, 400715, China.
Plant Cell Rep. 2025 Jan 22;44(2):32. doi: 10.1007/s00299-024-03421-6.
We identified a CXCXCPXC motif and 11 CLG genes that regulate epidermal development by interacting with homeodomain leucine-zipper IV family proteins in Arabidopsis. Zinc finger proteins (ZFPs), the key regulators of plant growth and development, can be categorized based on the sequence patterns of zinc finger motifs. Here, by aligning the amino acid sequences of CFL1, AtCFL1, AtCFL2, GIRl, and GIR2, we identified the CXCXCPXC motif in their C-terminus, which differs from all the previously characterized canonical zinc finger motifs. A total of 11 non-canonical ZFPs were identified in Arabidopsis based on the pattern and we named these genes as the CLG family genes (CFL1-like genes). We further established that the family genes are randomly distributed on five chromosomes, containing two conserved motifs and possess various cis-acting elements associated with plant hormones, stress responses and tissue regulation. Moreover, the family genes exhibit a wide range of expression profiles, and all of these proteins have nuclear localization signals. They may affect epidermal development through interactions with homeodomain leucine-zipper IV (HD-ZIP IV) family proteins. Overall, these findings comprehensively characterize CLG family genes and lay a foundation for further elaborating their biological functions in plant growth and development.
我们在拟南芥中鉴定出一个CXCXCPXC基序和11个通过与同源异型域亮氨酸拉链IV家族蛋白相互作用来调控表皮发育的CLG基因。锌指蛋白（ZFPs）是植物生长发育的关键调节因子，可根据锌指基序的序列模式进行分类。在此，通过比对CFL1、AtCFL1、AtCFL2、GIR1和GIR2的氨基酸序列，我们在它们的C端鉴定出了CXCXCPXC基序，该基序不同于所有先前已表征的典型锌指基序。基于该模式，在拟南芥中总共鉴定出11个非典型锌指蛋白，并将这些基因命名为CLG家族基因（CFL1样基因）。我们进一步确定，这些家族基因随机分布在五条染色体上，包含两个保守基序，并拥有与植物激素、应激反应和组织调控相关的各种顺式作用元件。此外，这些家族基因表现出广泛的表达谱，并且所有这些蛋白都具有核定位信号。它们可能通过与同源异型域亮氨酸拉链IV（HD-ZIP IV）家族蛋白相互作用来影响表皮发育。总体而言，这些发现全面地刻画了CLG家族基因，并为进一步阐述它们在植物生长发育中的生物学功能奠定了基础。
