通过形态学改变和中性红吸收在体外测定毒性。
Toxicity determined in vitro by morphological alterations and neutral red absorption.
作者信息
Borenfreund E, Puerner J A
出版信息
Toxicol Lett. 1985 Feb-Mar;24(2-3):119-24. doi: 10.1016/0378-4274(85)90046-3.
PMID:3983963
Abstract
A method is described which combines the use of a visual morphological cytotoxicity assay with a quantitative neutral red (NR) spectrophotometric test, for the assessment of the effect of toxic agents on 3T3 cells in culture. These sensitive and reproducible assays lend themselves to a screening procedure of potential toxicants which can help reduce the use of animals for toxicity testing.
摘要
本文描述了一种方法，该方法将视觉形态学细胞毒性试验与定量中性红（NR）分光光度法相结合，用于评估有毒试剂对培养的3T3细胞的影响。这些灵敏且可重复的试验适用于潜在毒物的筛选程序，有助于减少毒性测试中动物的使用。
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