Shi Anyuan, Guo Li, Gu Chunning, Zhan Yunni, Zhou Xuelian, Cheng Wei
Department of Dental Implantology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China.
Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, China.
Front Bioeng Biotechnol. 2025 Jan 7;12:1521223. doi: 10.3389/fbioe.2024.1521223. eCollection 2024.
Lignin is a naturally derived biomacromolecule with excellent biocompatibility and the potential for biomedical application. For the first time, this study isolated nanosized lignin microspheres (LMSs) directly from wheat straw with a polyol-based deep eutectic solvent. The size of these LMSs can be regulated by changing the isolation parameters, ranging from 90 nm to 330 nm. The structures of these LMSs were comprehensively investigated by SEM, gel permeation chromatography (GPC), HSQC NMR, and P NMR, which explained the formation mechanism of the hydrophobicity-induced self-assembly. The LMSs show good antioxidation of 52.99%-76.26% toward DPPH. biomedical tests further revealed that the LMSs at concentrations <25 μg/mL had good biocompatibility toward gingival mesenchymal stem cells (GMSCs) and jaw bone marrow mesenchymal stem cells (JBMMSCs), with a low apoptosis rate, outperforming other lignin materials. The presented results highlighted the application of the nanosized LMSs as a potential biomaterial in oral tissue regeneration.
木质素是一种天然衍生的生物大分子，具有优异的生物相容性和生物医学应用潜力。本研究首次使用基于多元醇的低共熔溶剂直接从小麦秸秆中分离出纳米级木质素微球（LMSs）。这些LMSs的尺寸可以通过改变分离参数进行调节，范围从90纳米到330纳米。通过扫描电子显微镜（SEM）、凝胶渗透色谱法（GPC）、异核单量子相干核磁共振（HSQC NMR）和磷核磁共振（P NMR）对这些LMSs的结构进行了全面研究，解释了疏水性诱导自组装的形成机制。LMSs对二苯基苦味酰基自由基（DPPH）显示出52.99%-76.26%的良好抗氧化性。生物医学测试进一步表明，浓度<25μg/mL的LMSs对牙龈间充质干细胞（GMSCs）和颌骨骨髓间充质干细胞（JBMMSCs）具有良好的生物相容性，凋亡率低，优于其他木质素材料。所呈现的结果突出了纳米级LMSs作为口腔组织再生潜在生物材料的应用。
