Qiu Daiyu, Wang Xue, Jiang Kan, Gong Gaoxia, Bao Fang
College of Agronomy, Gansu Agricultural University, Gansu, China.
Gansu Key Laboratory of Arid Habitat Crop Science, Gansu Agricultural University, Gansu, China.
Front Plant Sci. 2025 Jan 7;15:1452090. doi: 10.3389/fpls.2024.1452090. eCollection 2024.
is a perennial medicinal plant. It's generally cultivated for three years, and should avoid long-term continuous cultivation. However, unreasonable crop rotation and extensive fertilization are common in cultivation, which leads to the imbalance of soil microflora structure, and the obstacle of continuous cropping are becoming increasingly serious. Some microbial fertilizers such as , and complex microbial agent have the advantage of regulating soil microbial community structure and improving the soil environment. Therefore, these three kinds of microbial fertilizers were applied to and their effects on soil microorganisms of were studied.
Combine microbial fertilizers with conventional fertilization for continuous cropping and rotating . High-throughput sequencing technology was used to determine soil microbial richness, diversity and distribution of community structure in rotating and continuous cropping .
Continuous cropping reduced soil bacterial diversity by 7.56% and increased fungal richness by 17.01% compared with crop rotation. However, after the application of microbial fertilizers, the fungal richness and diversity of continuous cropping were significantly reduced by 4.76%20.96%, and the soil bacterial diversity of continuous cropping and rotating was significantly increased by 7.22%12.03% and 6.75%11.69% compared with the respective controls, respectively. Continuous cropping and rotating soil dominant bacteria mainly include and , and the dominant fungi include and . The activity process of these microbial communities was mainly through carbohydrate metabolism and amino acid synthesis pathway in metabolism. The complex microbial agent significantly increased the relative abundance of soil dominant bacteria communities of continuous cropping and rotating by 3.1111.54 percentage points, and significantly reduced the relative abundance of soil dominant fungal communities of continuous cropping by 1.57~8.93 percentage points, compared with the control. Of the three microbial fertilizers, the complex microbial agent had the most significant effects on optimizing the soil microbial community structure of continuous cropping and rotating . Conclusion: the application effect of different microbial fertilizers in continuous cropping was better than crop rotation, and the application effect of complex microbial agent was the best, which has more application value and development prospect in the cultivation management of .
[植物名称]是一种多年生药用植物。通常种植三年，应避免长期连作。然而，在种植过程中，不合理的轮作和粗放施肥较为常见，这导致土壤微生物群落结构失衡，连作障碍日益严重。一些微生物肥料如[微生物肥料名称1]、[微生物肥料名称2]和复合微生物菌剂具有调节土壤微生物群落结构和改善土壤环境的优势。因此，将这三种微生物肥料施用于[植物名称]，研究其对[植物名称]土壤微生物的影响。
将微生物肥料与常规施肥相结合用于连作和轮作的[植物名称]。采用高通量测序技术测定轮作和连作[植物名称]土壤微生物的丰富度、多样性及群落结构分布。
与轮作相比，连作使[植物名称]土壤细菌多样性降低了7.56%，真菌丰富度增加了17.01%。然而，施用微生物肥料后，连作[植物名称]的真菌丰富度和多样性显著降低了4.76%20.96%，连作和轮作[植物名称]的土壤细菌多样性分别比各自对照显著增加了7.22%12.03%和6.75%11.69%。连作和轮作[植物名称]土壤优势细菌主要包括[细菌名称1]和[细菌名称2]，优势真菌包括[真菌名称1]和[真菌名称2]。这些微生物群落的活性过程主要通过代谢中的碳水化合物代谢和氨基酸合成途径进行。与对照相比，复合微生物菌剂使连作和轮作[植物名称]土壤优势细菌群落的相对丰度显著增加了3.1111.54个百分点，使连作[植物名称]土壤优势真菌群落的相对丰度显著降低了1.57~8.93个百分点。在这三种微生物肥料中，复合微生物菌剂对优化连作和轮作[植物名称]土壤微生物群落结构的效果最显著。结论：不同微生物肥料在[植物名称]连作中的施用效果优于轮作，复合微生物菌剂的施用效果最佳，在[植物名称]栽培管理中具有更高的应用价值和发展前景。
