Fries Lisa M, Montrazi Elton T, Allouche-Arnon Hyla, Opazo Felipe, Bar-Shir Amnon, Frydman Lucio, Glöggler Stefan
NMR Signal Enhancement Group, Max Planck Institute for Multidisciplinary Sciences, 37077 Göttingen, Germany.
Center for Biostructural Imaging of Neurodegeneration of the University Medical Center, 37075 Göttingen, Germany.
Nano Lett. 2025 Feb 5;25(5):1758-1764. doi: 10.1021/acs.nanolett.4c03036. Epub 2025 Jan 22.
Deuterium (H) MRI is an emerging tool for noninvasive imaging. We explore the integration of H MRI with deuterated multifunctional nanopolymers for deuterated particle imaging (DPI). To this end, amine-terminated G5-polyamidoamine (PAMAM) dendrimers were labeled with deuterated acetyl surface groups, leading to highly H-loaded bioparticles, making them ideal for imaging studies. The accumulation of ∼5 nm PAMAM dendrimers in the kidneys could then be seen by H MRI with high submillimeter resolution. The natural abundance HDO signal provided an internal concentration reference to these measurements, leading to quantitative dynamic maps showing distinct nanopolymer uptakes within the renal compartments. Further, these nanopolymers allowed us to obtain maps of activity in the lymph nodes in an inflammatory rodent leg model, demonstrating these deuterated nanopolymers' potential as a novel class of contrast agents for the quantitative mapping of physiological processes.
氘（H）磁共振成像（MRI）是一种新兴的无创成像工具。我们探索将H MRI与氘代多功能纳米聚合物相结合用于氘代粒子成像（DPI）。为此，用氘代乙酰表面基团标记胺端基G5-聚酰胺-胺（PAMAM）树枝状大分子，得到高H负载的生物粒子，使其成为成像研究的理想选择。然后通过具有高亚毫米分辨率的H MRI可以看到约5纳米的PAMAM树枝状大分子在肾脏中的积累。天然丰度的HDO信号为这些测量提供了内部浓度参考，从而得到定量动态图，显示肾小室内不同的纳米聚合物摄取情况。此外，这些纳米聚合物使我们能够在炎症性啮齿动物腿部模型中获得淋巴结的活性图，证明这些氘代纳米聚合物作为一类新型造影剂用于生理过程定量映射的潜力。
